LOCUS       sequence-286833-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3430
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(668..692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             700..741
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ggactctcgg gcgccgcgca
       61 gtagccggcc tcctggcgtc acccagcccg gcccaggccc agaccctcac ccgggtcccg
      121 cggccggcag agttggcccc actctgcggc cgccgtggcc tgcgcaccga catcgatgcg
      181 acctgcacgc cccgccgcgc aagttcgaac caacgtggcc tcaaccagat ttggaatgtc
      241 aaaaagcaga gtgtctattt gatgaatttg aggaaatctg gaactttggg ccacccaggc
      301 tctctagatg agaccaccta tgaaagacta gcagaggaaa cgctggactc tttagcagag
      361 ttttttgaag accttgcaga caagccatac acgtttgagg actatgatgt ctcctttggg
      421 agtggtgtct taactgtcaa actgggtgga gatctaggaa cctatgtgat caacaagcag
      481 acgccaaaca agcaaatctg gctatcttct ccatccagtg gacctaagcg ttatgactgg
      541 actgggaaaa actgggtgta ctcccacgac ggcgtgtccc tccatgagct gctggccgca
      601 gagctcacta aagccttaaa aaccaaactg gacttgtctt ccttggccta ttccggaaaa
      661 gatgcttgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      721 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      781 tcttggcttt atatatcttg tggaaaggac gaacattgca gaaactagat caatgc
//
