LOCUS       sequence-286836-        3173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3431
FEATURES             Location/Qualifiers
     source          1..3173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3005..3029)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3037..3078
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttcg ccaccatgga gaccaaaggc
       61 taccacagtc tccctgaagg tctagatatg gaaagacggt ggggtcaagt ttctcaggct
      121 gtggagcgtt cttccctggg acctacagag aggaccgatg agaataacta catggagatt
      181 gtcaacgtaa gctgtgtttc cggtgctatt ccaaacaaca gtactcaagg aagcagcaaa
      241 gaaaaacaag aactactccc ttgccttcag caagacaata atcggcctgg gattttaaca
      301 tctgatatta aaactgagct ggaatctaag gaactttcag caactgtagc tgagtccatg
      361 ggtttatata tggattctgt aagagatgct gactattcct atgagcagca gaaccaacaa
      421 ggaagcatga gtccagctaa gatttatcag aatgttgaac agctggtgaa attttacaaa
      481 ggaaatggcc atcgtccttc cactctaagt tgtgtgaaca cgcccttgag atcatttatg
      541 tctgactctg ggagctccgt gaatggtggc gtcatgcgcg ccgttgttaa aagccctatc
      601 atgtgtcatg agaaaagccc gtctgtttgc agccctctga acatgacatc ttcggtttgc
      661 agccctgctg gaatcaactc tgtgtcctcc accacagcca gctttggcag ttttccagtg
      721 cacagcccaa tcacccaggg aactcctctg acatgctccc ctaatgttga aaatcgaggc
      781 tccaggtcgc acagccctgc acatgctagc aatgtgggct ctcctctctc aagtccgtta
      841 agtagcatga aatcctcaat ttccagccct ccaagtcact gcagtgtaaa atctccagtc
      901 tccagtccca ataatgtcac tctgagatcc tctgtgtcta gccctgcaaa tattaacaac
      961 tcaaggtgct ctgtttccag cccttcgaac actaataaca gatccacgct ttccagtccg
     1021 gcagccagta ctgtgggatc tatctgtagc cctgtaaaca atgccttcag ctacactgct
     1081 tctggcacct ctgctggatc cagtacattg cgggatgtgg ttcccagtcc agacacgcag
     1141 gagaaaggtg ctcaagaggt cccttttcct aagactgagg aagtagagag tgccatctca
     1201 aatggtgtga ctggccagct taatattgtc cagtacataa aaccagaacc agatggagct
     1261 tttagcagct catgtctagg aggaaatagc aaaataaatt cggattcttc attctcagta
     1321 ccaataaagc aagaatcaac caagcattca tgttcaggca cctcttttaa agggaatcca
     1381 acagtaaacc cgtttccatt tatggatggc tcgtattttt cctttatgga tgataaagac
     1441 tattattccc tatcaggaat tttaggacca cctgtgcccg gctttgatgg taactgtgaa
     1501 ggcagcggat tcccagtggg tattaaacaa gaaccagatg atgggagcta ttacccagag
     1561 gccagcatcc cttcctctgc tattgttggg gtgaattcag gtggacagtc cttccactac
     1621 aggattggtg ctcaaggtac aatatcttta tcacgatcgg ctagagacca atctttccaa
     1681 cacctgagtt cctttcctcc tgtcaatact ttagtggagt catggaaatc acacggcgac
     1741 ctgtcgtcta gaagaagtga tgggtatccg gtcttagaat acattccaga aaatgtatca
     1801 agctctactt tacgaagtgt ttctactgga tcttcaagac cttcaaaaat atgtttggtg
     1861 tgtggggatg aggcttcagg atgccattat ggggtagtca cctgtggcag ctgcaaagtt
     1921 ttcttcaaaa gagcagtgga agggcaacac aactatttat gtgctggaag aaatgattgc
     1981 atcattgata agattcgacg aaagaattgt cctgcttgca gacttcagaa atgtcttcaa
     2041 gctggaatga atttaggagc acgaaagtca aagaagttgg gaaagttaaa agggattcac
     2101 gaggagcagc cacagcagca gcagccccca cccccacccc cacccccgca aagcccagag
     2161 gaagggacaa cgtacatcgc tcctgcaaaa gaaccctcgg tcaacacagc actggttcct
     2221 cagctctcca caatctcacg agcgctcaca ccttcccccg ttatggtcct tgaaaacatt
     2281 gaacctgaaa ttgtatatgc aggctatgac agctcaaaac cagatacagc cgaaaatctg
     2341 ctctccacgc tcaaccgctt agcaggcaaa cagatgatcc aagtcgtgaa gtgggcaaag
     2401 gtacttccag gatttaaaaa cttgcctctt gaggaccaaa ttaccctaat ccagtattct
     2461 tggatgtgtc tatcatcatt tgccttgagc tggagatcgt acaaacatac gaacagccaa
     2521 tttctctatt ttgcaccaga cctagtcttt aatgaagaga agatgcatca gtctgccatg
     2581 tatgaactat gccaggggat gcaccaaatc agccttcagt tcgttcgact gcagctcacc
     2641 tttgaagaat acaccatcat gaaagttttg ctgctactaa gcacaattcc aaaggatggc
     2701 ctcaaaagcc aggctgcatt tgaagaaatg aggacaaatt acatcaaaga actgaggaag
     2761 atggtaacta agtgtcccaa caattctggg cagagctggc agaggttcta ccaactgacc
     2821 aagctgctgg actccatgca tgacctggtg agcgacctgc tggaattctg cttctacacc
     2881 ttccgagagt cccatgcgct gaaggtagag ttccccgcaa tgctggtgga gatcatcagc
     2941 gaccagctgc ccaaggtgga gtcggggaac gccaagccgc tctacttcca ccggaagtga
     3001 taggacccag ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     3061 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3121 tggctttata tatcttgtgg aaaggacgag taacgagtta cctggttaac tcc
//
