LOCUS       sequence-286842-        1329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3433
FEATURES             Location/Qualifiers
     source          1..1329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1193..1234
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ccgcctcggc ccctgctgcg
       61 gagggggagg gaacccccac ccagcccgcg tccgagaaag aacccgaaat gcccggtccc
      121 agagaggaga gcgaggagga agaggacgag gacgacgagg aggaggagga ggaggaaaaa
      181 gaaaagagtc tcatcgtgga aggcaagagg gaaaagaaaa aagtagagag gttgacaatg
      241 caagtctctt ccttacagag agagccattt acaattgcac aaggaaaggg gcagaaactt
      301 tgtgaaattg agaggataca tttttttcta agtaagaaga aaaccgatga acttagaaat
      361 ctacacaaac tgctttacaa caggccaggc actgtgtcct cattaaagaa gaatgtgggt
      421 cagttcagtg gctttccatt tgaaaaagga agtgtccaat ataaaaagaa ggaagaaatg
      481 ttgaaaaaat ttagaaatgc catgttaaag agcatctgtg aggttcttga tttggagaga
      541 tcaggtgtaa atagtgaact agtgaagagg atcttgaatt tcttaatgca tccaaagcct
      601 tctggcaaac cattgccgaa atctaaaaaa acttgtagca aaggcagtaa aaaggaacgg
      661 aacagttctg gaatggcaag gaaggctaag cgaaccaaat gtcctgaaat tctgtcagat
      721 gaatctagta gtgatgaaga tgaaaagaaa aacaaggaag agtcttcaga tgatgaagat
      781 aaagaaagtg aagaggagcc accaaaaaag acagccaaaa gagaaaaacc taaacagaaa
      841 gctacttcta aaagtaaaaa atctgtgaaa agtgccaatg ttaagaaagc agatagcagc
      901 accaccaaga agaatcaaaa cagttccaaa aaagaaagtg agtctgagga tagttcagat
      961 gatgaacctt taattaaaaa gttgaagaaa ccccctacag atgaagagtt aaaggaaaca
     1021 ataaagaaat tactggccag tgctaacttg gaagaagtca caatgaaaca gatttgcaaa
     1081 aaggtctatg aaaattatcc tacttatgat ttaactgaaa gaaaagattt cataaaaaca
     1141 actgtaaaag agctaatttc gcccaacttc cttgtacaaa gtggttgata tcggtaagcc
     1201 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga
     1261 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagttct acctatcgga
     1321 ggattcagc
//
