LOCUS       sequence-286845-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3434
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1721..1745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1753..1794
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tcacatccca gggatcagtg
       61 tcgtttaggg atgtgactgt gggcttcact caagaggagt ggcagcatct ggaccctgct
      121 cagaggaccc tgtacaggga tgtgatgctg gagaactaca gccaccttgt ctcagtaggg
      181 tattgcattc ctaaaccaga agtgattctc aagttggaga aaggcgagga gccatggata
      241 ttagaggaaa aatttccaag ccagagtcat ctggaattaa ttaataccag tagaaactat
      301 tcaataatga agttcaatga gtttaacaaa ggtggaaaat gtttctgtga tgaaaagcat
      361 gaaataattc attctgaaga ggaaccttct gaatataata aaaatgggaa cagcttctgg
      421 ctgaatgaag acctcatttg gcatcagaaa attaaaaatt gggaacaatc ttttgaatac
      481 aatgaatgtg ggaaagcttt ccctgagaat tcactcttcc ttgtacataa gagaggttac
      541 acaggacaga aaacctgcaa atatactgaa catgggaaaa cctgtgatat gtcatttttc
      601 atcactcatc agcaaacaca tccaagagaa aaccactatg gtaatgaatg tggagaaaat
      661 atctttgagg aatccattct ccttgaacat cagagtgttt acccattcag ccagaagtta
      721 aatctcactc caattcagag aacccactca attaacaata ttattgaata taatgagtgt
      781 ggaacatttt tcagtgaaaa attagtcctt catttacaac agagaacaca tacaggagaa
      841 aaaccttatg aatgtcatga atgtggaaaa accttcaccc agaagtcagc ccacacaaga
      901 catcagagaa cacacacagg gggaaaaccc tatgaatgtc atgaatgtgg gaagaccttc
      961 tataagaatt cagacctcat taaacatcaa agaattcata caggggagag accttatgga
     1021 tgtcatgaat gtgggaaatc cttcagtgaa aagtcaaccc ttactcaaca tcaaagaacg
     1081 cacacagggg agaaaccata tgaatgtcat gaatgtggga aaaccttctc atttaagtca
     1141 gtccttactg tgcatcagaa aacacacaca ggggagaagc cctatgaatg ctatgcatgt
     1201 gggaaagcct ttctcagaaa atcagacctc attaaacatc aaagaataca cacaggtgaa
     1261 aaaccttatg aatgtaatga atgtgggaag tcattctctg agaagtcaac ccttactaaa
     1321 catctaagaa ctcacacagg tgagaaacct tatgaatgta ttcagtgtgg aaaatttttc
     1381 tgctactact ccggtttcac agaacatctg agaagacaca caggggagaa accttttgga
     1441 tgtaatgaat gtgggaaaac cttccgtcag aagtcggccc taattgttca ccagagaact
     1501 catataagac agaaacccta tggatgtaat caatgtggaa aatcattctg tgtgaagtca
     1561 aaactcattg cacatcatag aacacacaca ggggagaaac cctatgaatg taatgtttgt
     1621 ggaaaatcat tctatgttaa gtcaaaacta actgtacatc agagaataca cttggggaga
     1681 aaccctataa atgtagtaaa cgagggaaat tactctgggt gcccaacttt cttgtacaaa
     1741 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgaccgct ccctgaaggt ccctatccc
//
