LOCUS       sequence-286848-        3467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3435
FEATURES             Location/Qualifiers
     source          1..3467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1417..1428)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3299..3323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3331..3372
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cggacagcga ggacagcaac
       61 ttttccgagg aggaggacag cgagcgcagc agtgacggcg aggaggccga ggtagacgaa
      121 gagcggcgga gtgcagcggg cagtgagaaa gaagaagagc ctgaggacga agaggaggag
      181 gaagaggagg aggaatatga tgaggaagag gaagaagaag atgatgaccg accccccaag
      241 aaaccccgcc atggaggctt cattctggac gaggctgatg ttgacgatga gtatgaggac
      301 gaggaccagt gggaggatgg agcagaggac attctagaga aagaagagat tgaagcctcc
      361 aatatcgata atgttgtcct ggatgaagat cgttctgggg ctcgccgcct gcaaaacctc
      421 tggagggacc agcgagaaga agaactgggc gagtattaca tgaagaaata cgccaagtca
      481 tctgtgggag agacggtgta tggaggatct gatgagctct cagacgacat cacccagcag
      541 cagctgctcc caggagtcaa ggatcccaat ctgtggactg tcaaatgtaa gattggggag
      601 gaacgggcca cggccatttc cttgatgcgc aagttcattg cctaccagtt cacagacacg
      661 cccctgcaga tcaagtcagt agtggcacca gagcatgtga agggctacat ctacgtggag
      721 gcctacaagc agacccacgt gaagcaggcc attgaggggg tgggcaacct gcggcttggc
      781 tactggaacc agcagatggt gcccatcaag gagatgacag acgtgctcaa agtggtgaag
      841 gaggtggcca acctgaaacc aaagtcctgg gtccgcctca agcggggcat ctacaaggat
      901 gacattgctc aggtggacta cgtggagccc agccagaaca ccatctccct gaagatgatc
      961 ccacgcatcg actacgatcg catcaaggcc cgcatgagct tgaaagactg gtttgccaaa
     1021 aggaagaagt ttaagcggcc tccacagagg ctgtttgatg ctgagaagat caggtccctg
     1081 gggggtgatg ttgcctctga tggtgacttc ctcatctttg aggggaaccg ttacagccgg
     1141 aagggctttc tgttcaagag cttcgccatg tctgctgtga tcacggaggg tgtgaagcca
     1201 acactctctg agctggaaaa gtttgaggac cagccagagg gcattgacct ggaggtggtg
     1261 actgagagca cagggaagga gcgggagcac aacttccaac ctggggacaa cgtggaggtc
     1321 tgtgagggtg agctcatcaa cctgcagggc aagatcctca gcgtggatgg caacaagatc
     1381 accatcatgc ccaagcatga ggacctcaag gacatgttgg agttcccagc ccaggaactt
     1441 agaaaatact tcaagatggg ggaccacgtg aaggtgattg ctggccgatt cgagggcgac
     1501 acaggcctca ttgtgcgggt ggaggagaat ttcgttatcc tgttctctga cctcaccatg
     1561 catgagctga aggtgctccc ccgggacctg cagctctgct cagagacagc atcaggtgtg
     1621 gatgttgggg gccagcatga atggggcgag ctggtgcagc tggatcccca gactgtgggt
     1681 gtcatcgtgc gactagaacg ggagaccttc caggtgctga acatgtacgg gaaggtggtg
     1741 actgtcagac atcaggctgt gacccggaag aaggacaacc gctttgctgt ggccttggac
     1801 tcagagcaga acaacatcca tgtgaaagac atcgttaagg tcattgatgg cccccactca
     1861 ggccgagaag gggagattcg ccatctcttc cgaagcttcg ccttcctaca ttgcaagaaa
     1921 ctggtggaga acgggggcat gtttgtctgc aagacccgcc acctggtgct ggctgggggc
     1981 tcaaagcccc gtgatgtgac caacttcacc gtgggtggct ttgcgcctat gagtccccgg
     2041 atcagcagcc ccatgcaccc cagtgctgga ggtcagcgtg gcggctttgg tagcccaggt
     2101 ggcggcagtg gtggcatgag caggggccgg ggccggaggg acaacgaact catcggccag
     2161 accgtgcgca tctcccaggg gccctacaaa ggctacatcg gtgtggtgaa agatgccaca
     2221 gagtccacgg cccgtgtgga gctgcactcc acctgccaga ccatctctgt ggaccgtcag
     2281 cggctcacca cggtgggctc acggcgcccg ggcggcatga cctcgaccta tgggaggacg
     2341 cccatgtatg gctcccagac gcccatgtat ggctctggct cccgaacacc catgtacggc
     2401 tcacagacac ccctccagga tggtagccgc accccacact acggctcaca gacgcccctg
     2461 catgatggca gccgcactcc tgcccagagt ggggcctggg accccaacaa ccccaacacg
     2521 ccgtcacggg ctgaggaaga atatgagtat gctttcgatg atgagcccac cccgtccccg
     2581 caggcctatg ggggaacccc caatccccaa acacctggct acccagaccc ctcgtcccca
     2641 caggtcaacc cacaatacaa cccgcagacg ccagggacgc cggccatgta caacacagac
     2701 cagttctctc cctatgctgc cccctcccca caaggttcct accagcccag ccccagcccc
     2761 cagagctacc accaggtggc gccaagccca gcaggctacc agaataccca ctccccagcc
     2821 agctaccacc ctacaccgtc gcccatggcc tatcaggcta gccccagccc gagccccgtt
     2881 ggctacagtc ctatgacacc tggagctccc tcccctggtg gctacaaccc acacacgcca
     2941 ggctcaggca tcgagcagaa ctccagcgac tgggtaacca ctgacattca ggtgaaggtg
     3001 cgggacacct acctggatac acaggtggtg ggacagacag gtgtcatccg cagtgtcacg
     3061 gggggcatgt gctctgtgta cctgaaggac agtgagaagg ttgtcagcat ttccagtgag
     3121 cacctggagc ctatcacccc caccaagaac aacaaggtga aagtgatcct gggcgaggat
     3181 cgggaagcca cgggcgtcct actgagcatt gatggtgagg atggcattgt ccgtatggac
     3241 cttgatgagc agctcaagat cctcaacctc cgcttcctgg ggaagctcct ggaagcctgc
     3301 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     3361 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3421 tatatatctt gtggaaagga cgacttctgc cctcgcccgt taaggca
//
