LOCUS       sequence-286851-        2069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3436
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1901..1925)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1933..1974
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agtttcccgg aggaaatgac
       61 aattacctga cgatcacagg gccttcgcac cccttcctgt caggggccga gacattccat
      121 acaccaagct tgggtgatga ggaatttgaa atcccaccta tctccttgga ttctgatccc
      181 tcattggctg tctcagatgt ggttggccac tttgatgacc tggcagaccc ttcctcttca
      241 caggatggca gtttttcagc ccagtatggg gtccagacat tggacatgcc tgtgggcatg
      301 acccatggct tgatggagca gggcgggggg ctcctgagtg ggggcttgac catggacttg
      361 gaccactcta taggaactca gtatagtgcc aacccacctg ttacaattga tgtaccaatg
      421 acagacatga catctggctt gatggggcat agccagttga ccaccattga tcagtcagaa
      481 ctgagttccc agctgggttt gagcctaggg ggtggcacca tcctgccacc tgcccagtca
      541 cctgaagatc gtctttcaac caccccttca cctactagtt cacttcacga ggatggtgtt
      601 gaggatttcc ggaggcaact tcccagccag aagacagtcg tggtggaagc agggaaaaag
      661 cagaaggccc caaagaagag aaaaaagaaa gatcctaatg aacctcagaa accagtttca
      721 gcatatgctt tattctttcg tgatacacag gctgccatca agggacagaa tcctaatgcc
      781 acttttggtg aggtttcaaa aattgtggcc tccatgtggg atagtcttgg agaggagcaa
      841 aaacaggtat ataagaggaa aactgaggct gccaagaaag agtatctgaa ggcactggct
      901 gcttacaaag acaaccagga gtgtcaggcc actgtggaaa cagtggaatt ggatccagca
      961 ccaccatcac aaactccttc tccacctcct atggctactg ttgacccagc atctccagca
     1021 ccagcttcaa tagagccccc tgccctgtcc ccatccattg ttgttaactc caccctctca
     1081 tcctatgtgg caaaccaggc atcttctgga gctgggggtc agcccaatat caccaagttg
     1141 attattacca aacaaatgtt gccctcttct attactatgt ctcaaggagg gatggttact
     1201 gttatcccag ccacagtggt gacctcccga gggctccaac taggccaaac cagtacagct
     1261 actatccagc ccagtcaaca agcccagatt gtcactcggt cagtgttgca ggcagcagca
     1321 gctgctgctg ctgctgcttc tatgcaactg cctccacccc gactacagcc ccctccatta
     1381 caacagatgc cacagccccc gactcagcag caagttacca ttctgcagca gcctcctcca
     1441 ctccaggcca tgcaacagcc tccacctcag aaagttcgaa tcaatttaca gcaacagcct
     1501 cctcctctgc agatcaagag tgtgcctcta cccactttga aaatgcagac taccttagtc
     1561 ccaccaactg tggaaagtag tcctgagcgg cctatgaaca acagccctga ggcccataca
     1621 gtggaggcac cttctcctga gactatctgt gagatgatca cagatgtagt tcctgaggtt
     1681 gagtctcctt ctcagatgga tgttgaattg gtgagtgggt ctcctgtggc actctcaccc
     1741 cagcctcgat gtgtgaggtc tggttgtgag aaccctccca ttgtgagtaa ggactgggac
     1801 aatgaatact gcagcaatga gtgtgtggtg aagcactgca gggatgtatt cttggcctgg
     1861 gtagcctcta gaaattcaaa cacagtggtg tttgtgaaat acccaacttt cttgtacaaa
     1921 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1981 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2041 gacgactaaa agcttgagac ccagttatg
//
