LOCUS       sequence-286854-        2351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3437
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2183..2207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2215..2256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaacacatt gcaggggcca
       61 gtgtcattca aagatgtggc tgtggatttc acccaggagg agtggcagca gctggaccct
      121 gatgagaaga taacttacag ggatgtgatg ttggagaact atagccatct agtttctgtg
      181 ggatatgata ccaccaagcc aaacgtcatc attaagttgg agcagggaga ggagccgtgg
      241 ataatgggag gtgaatttcc atgtcaacat agtccagaag cctggagagt tgatgacctg
      301 atagagagaa tccaagaaaa cgaagacaaa cattcaaggc aagctgcttg tatcaatagc
      361 aaaaccctga ctgaagagaa agagaataca tttagtcaaa tttacatgga aacaagcctt
      421 gttccttcaa gcataatagc tcataattgt gtctcatgtg gaaagaattt agaatctatt
      481 tcgcaattaa ttagtagtga tggaagctat gctaggacaa aacctgatga gtgtaatgaa
      541 tgtgggaaaa catatcatgg agagaaaatg tgtgaattta atcaaaatgg ggatacctat
      601 tctcacaatg aagaaaatat tcttcagaaa attagtattt tggagaaacc ctttgaatat
      661 aatgaatgca tggaagcctt agacaatgag gctgttttta ttgctcataa gagagcttac
      721 ataggggaga agccctatga gtggaatgat tctggaccag acttcataca gatgtcaaat
      781 tttaatgcat atcagagatc acaaatggaa atgaagccct ttgaatgcag tgaatgtgga
      841 aaatccttct gtaaaaagtc aaaattcatc atccaccaga gggctcacac aggagagaaa
      901 ccttatgaat gtaatgtatg tgggaaatcc ttcagccaaa agggaaccct cactgtacat
      961 cggagatcac acttagagga gaagccctat aaatgtaatg aatgtgggaa aaccttttgt
     1021 cagaagttac acctcactca acacctaaga actcattcag gagagaaacc ctacgaatgt
     1081 agcgaatgtg ggaaaacctt ctgccaaaag acacatctca ccctgcacca gaggaatcat
     1141 tcaggagaga ggccctatcc atgtaacgaa tgtgggaaat ccttctcccg caagtctgct
     1201 ctcagtgacc atcagagaac tcacacagga gagaagcttt ataaatgtaa tgaatgtggg
     1261 aaatcctact accgaaagtc tactctgatt acacatcaga gaacacacac aggagagaag
     1321 ccctatcagt gtagcgagtg tgggaaattc ttttctcggg tgtcatacct cactatacat
     1381 tatagaagtc atttagaaga gaaaccctat gaatgtaatg aatgtggcaa aaccttcaat
     1441 ttaaattcag ccttcattag acatcggaaa gtacacacag aagagaaatc ccatgaatgt
     1501 agtgaatgtg gaaagttctc tcagttgtat ctcaccgacc atcatacagc tcatttagaa
     1561 gagaaaccct atgaatgtaa tgaatgtggg aaaaccttcc ttgtaaattc agccttcgat
     1621 gggcaccagc cacttccaaa aggggagaaa tcctatgaat gtaatgtatg tggaaagtta
     1681 ttcaatgagt tgtcatacta tactgaacat tatagaagtc attcagaaga gaaaccttat
     1741 ggatgtagcg aatgtgggaa aaccttttcc cataattcat ccctcttcag acatcaaaga
     1801 gtacacacag gcgagaaacc ctatgaatgt tacgaatgtg gaaaattctt ctctcagaaa
     1861 tcatatctca ctatacatca tcgaattcat tcaggagaga aaccctatga atgtagtaaa
     1921 tgtggaaaag tcttctctcg gatgtcaaac ctcactgtcc actacagaag ccattcagga
     1981 gagaaaccct atgaatgtaa tgaatgtggg aaagtctttt ctcagaagtc atacctcact
     2041 gtacactata gaactcattc aggagagaaa ccctatgaat gtaatgagtg tgggaaaaaa
     2101 ttccaccaca gatcagcctt caatagccat cagagaattc atagaagagg aaatatgaat
     2161 gtacttgatg tggaaaatct cttgccaact ttcttgtaca aagtggttga tatcggtaag
     2221 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     2281 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgactc tgttgagccc
     2341 cagagcagga a
//
