LOCUS       sequence-286857-        1517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3438
FEATURES             Location/Qualifiers
     source          1..1517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1349..1373)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1381..1422
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaacctga cgactttgat
       61 tctgaagaca aagagatatt aagctgggat attaatgatg tgaaactgcc acagaacgtg
      121 aaaaaaaccg actggttcca ggagtggcca gattcctatg ccaaacacat ctacagctcg
      181 gaggacaaga atgcgcagcg gcacctgagc agctgggcca tgcgcaatac caacaaccac
      241 aactcccgca tcctcaagaa gtcctgcctg ggtgtggtgg tgtgcggccg cgactgtctc
      301 gcagaggagg ggcgcaagat ctacctgaga cctgccatct gtgacaaggc ccggcagaag
      361 cagcagcgga aacgctgtcc caactgtgac gggcctctga agctcatccc ttgccgaggt
      421 catgggggct tcccggtcac caacttctgg aggcacgacg gacgctttat atttttccag
      481 tcaaagggag agcatgatca tccaaaacca gaaaccaagt tagaagctga ggcaagaaga
      541 gccatgaaga aagtgaacac agcaccttcc tccgtctcat tgagcctgaa ggggagcaca
      601 gagaccaggt ctcttccagg tgaaacacaa agtcagggga gtttaccttt aacttggtct
      661 ttccaggaag gcgtccaatt gcctggtagt tacagtggac atttaatagc taacactcct
      721 cagcagaact cactaaatga ttgcttttcc ttctccaaga gttatggtct gggaggaatc
      781 acagatctga ctgaccagac ttccactgtg gaccccatga agctctatga aaagcgcaaa
      841 ttgtccagta gcagaaccta cagtagtgga gacctgcttc ctccttctgc ctccggagtc
      901 tactctgatc atggcgatct acaagcgtgg agtaaaaatg ctgctttggg gagaaatcat
      961 cttgctgaca actgttattc caattatcct tttcctctga ccagctggcc ttgcagcttc
     1021 tctccttccc aaaactcttc agaacccttt taccagcagc ttccattgga gccacctgca
     1081 gccaaaactg gctgtccccc attatggcca aatccagcgg gtaatcttta tgaagagaaa
     1141 gtacatgtgg attttaacag ctacgtccag tctcctgcat accattcacc tcaagaagac
     1201 ccctttctct tcacctacgc ctctcatcct catcagcaat attcactgcc aagcaagagc
     1261 agcaaatggg attttgagga agaaatgaca tacttgggtt tggatcactg caacaatgat
     1321 atgcttctga acctgtgtcc tttgagattg ccaactttct tgtacaaagt ggttgatatc
     1381 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1441 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaattcgag
     1501 tttatctaag ttactta
//
