LOCUS       sequence-286860-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3439
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(890..914)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             922..963
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgagctcctt cgacctgccg
       61 gcgccctccc cacctcgctg cagcccccag ttccccagca tcggccagga gccccccgag
      121 atgaacctct actatgagaa cttcttccac ccacagggcg tgcccagccc tcagcggccc
      181 tccttcgagg ggggcggcga gtatggggcc acccccaacc cctacctctg gttcaacggg
      241 cccaccatga ccccgccacc ctacctgccc ggccccaacg ccagcccctt cctgccccag
      301 gcctatggag tgcagaggcc gctgctgccc agcgtgtcgg ggcttggggg gagcgacctg
      361 ggctggctgc ccatcccctc gcaggaggag ctgatgaagc tggtgcggcc accctattcc
      421 tactcggctc tcatcgccat ggccatccac ggggcacccg acaagcgcct cactctcagc
      481 cagatctacc agtacgtggc cgacaacttc cccttctaca acaagagcaa ggccggctgg
      541 cagaactcca tccgccacaa cctgtcgctc aacgactgct tcaagaaggt gccccgcgac
      601 gaggacgacc cggcctatgt gagcgggggg agccccacga gccacccctt ggtcacacca
      661 ggactgagcc ctgagcccag tgacaagacg gggcagaact cactgacctt caactccttc
      721 tccccgctca ccaacctcag caaccacagc ggtgggggtg actgggcgaa ccccatgccc
      781 accaacatgc tcagctacgg aggatctgtg ctcagccaat tcagccctca cttctacaac
      841 agtgtcaaca ccagtggtgt cctctacccc agggagggca ccgaggtctt gccaactttc
      901 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      961 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1021 tgtggaaagg acgagtgacc tcactatact gcggcaat
//
