LOCUS       sequence-286863-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3440
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(878..902)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             910..951
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgttgagtcc tgccaacggg
       61 gagcagctcc acctggtgaa ctatgtggag gactacctgg actccatcga gtccctgcct
      121 ttcgacttgc agagaaatgt ctcgctgatg cgggagatcg acgcgaaata ccaagagatc
      181 ctgaaggagc tagacgagtg ctacgagcgc ttcagtcgcg agacagacgg ggcgcagaag
      241 cggcggatgc tgcactgtgt gcagcgcgcg ctgatccgca gccaggagct gggcgacgag
      301 aagatccaga tcgtgagcca gatggtggag ctggtggaga accgcacgcg gcaggtggac
      361 agccacgtgg agctgttcga ggcgcagcag gagctgggcg acacagcggg caacagcggc
      421 aaggctggcg cggacaggcc caaaggcgag gcggcagcgc aggctgacaa gcccaacagc
      481 aagcgctcac ggcggcagcg caacaacgag aaccgtgaga acgcgtccag caaccacgac
      541 cacgacgacg gcgcctcggg cacacccaag gagaagaagg ccaagacctc caagaagaag
      601 aagcgctcca aggccaaggc ggagcgagag gcgtcccctg ccgacctccc catcgacccc
      661 aacgaaccca cgtactgtct gtgcaaccag gtctcctatg gggagatgat cggctgcgac
      721 aacgacgagt gccccatcga gtggttccac ttctcgtgcg tggggctcaa tcataaaccc
      781 aagggcaagt ggtactgtcc caagtgccgg ggggagaacg agaagaccat ggacaaagcc
      841 ctggagaaat ccaaaaaaga gagggcttac aacaggttgc caactttctt gtacaaagtg
      901 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      961 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1021 gagtatctaa ctcttttttg aattcg
//
