LOCUS       sequence-286866-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3441
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(965..989)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             997..1038
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgatcaggg ttgccctcgg
       61 tgtaagacca ccaaatatcg gaacccctcc ttgaagctga tggtgaatgt gtgcggacac
      121 actctctgtg aaagttgtgt agatttactg tttgtgagag gagctggaaa ctgccctgag
      181 tgtggtactc cactcagaaa gagcaacttc agggtacaac tctttgaaga tcccactgtt
      241 gacaaggagg ttgagatcag gaaaaaagtg ctaaagatat acaataaaag ggaagaagat
      301 tttcctagtc taagagaata caatgatttc ttggaagaag tggaagaaat tgttttcaac
      361 ttgaccaaca atgtggattt ggacaacacc aaaaagaaaa tggagatata ccaaaaggaa
      421 aacaaagatg ttattcagaa aaataaatta aagctgactc gagaacagga agaactggaa
      481 gaagctttag aagtggaacg acaggaaaat gaacaaagaa gattatttat acaaaaagaa
      541 gaacaactgc agcagattct aaaaaggaag aataagcagg cttttttaga tgagctggag
      601 agttctgatc tccctgttgc tctgcttttg gctcagcata aagatagatc tacccaatta
      661 gaaatgcaac ttgagaaacc caaacctgta aaaccagtga cgttttccac aggcatcaaa
      721 atgggtcaac atatttcact ggcacctatt cacaagcttg aagaagctct gtatgaatac
      781 cagccactgc agatagagac atatggacca catgttcctg agcttgagat gctaggaaga
      841 cttgggtatt taaaccatgt cagagctgcc tcaccacagg accttgctgg aggctatact
      901 tcttctcttg cttgtcacag agcactacag gatgcattca gtgggctttt ctggcagccc
      961 agttacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1021 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1081 tggctttata tatcttgtgg aaaggacgaa agcggaacgt ctccgggccg gcg
//
