LOCUS       sequence-286869-        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3442
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1001..1025)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1033..1074
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgcagggtg gctgacaacc
       61 tggtcacaga actcagtaac cttccaggaa gtggcagtgg acttttccca ggaggagtgg
      121 gcattgctgg accctgctca aaaaaatcta tacaaagatg tgatgctgga gaactttagg
      181 aacctggcct cagtagggta tcagctctgc agacacagtc tgatctccaa ggtggatcaa
      241 gaacagctga agacagatga aagaggaatt ttacaaggtg actgtgcaga ctgggaaact
      301 caacttaaac caaaagatac aattgctatg cagaacattc ctgggggaaa aacatccaat
      361 ggcataaaca cgaactgtgt cagaactcac tctggagaga tgccctatga atgcagtgac
      421 tgtgggaaag ccttcatttt tcagtcttcc cttaagaaac acatgagatc tcatactgga
      481 gagaagcctt atgagtgtga tcactgtgga aaatccttta gccagagctc tcatctgaat
      541 gtgcacaaaa gaactcacac tggagagaaa ccctatgact gtaaggaatg tgggaaggct
      601 ttcactgttc cttcatccct tcagaaacat gtgagaaccc acactggaga gaaaccctat
      661 gaatgcagtg actgtggaaa agccttcatc gatcagtcat cccttaagaa acacacacgc
      721 tctcacactg gagagaagcc ttatgagtgt aaccagtgtg gaaagtcctt cagcacaggc
      781 tcttacctta ttgtgcacaa gagaactcac actggtgaga aaacctatga gtgtaaagaa
      841 tgtgggaagg cctttaggaa ttcctcttgc ctgagggtac acgtgagaac tcacactgga
      901 gagaagcctt ataaatgtat tcagtgtgaa aaagccttta gcacaagcac taaccttata
      961 atgcacaagc gaatccacaa tggccagaaa ctccatgaat gcccaacttt cttgtacaaa
     1021 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1081 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1141 gacgaaaggc catttatgtt gagaagcct
//
