LOCUS       sequence-28687-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR/shp16 (w98-1)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..104
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        182..200
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     182..199
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        461..709
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     461..481
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     632..651
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gggcccaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa
       61 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctttaaag gaaccaattc
      121 agtcgaggtt atcaaacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      181 gatttaggtg acactataga atacaagctt ggctgcaggt cgacggatcc ccccgagtcc
      241 aacacccgtg ggaatcccat gggcaccatg gcccctcgct ccaaaaatgc tttcgcgtcg
      301 cgcagacact gctcggtagt ttcggggatc agcgtttgag taagagcccg cgtctgaacc
      361 ctccgcgccg ccccggcccc agtggaaaga cgcgcaggca aaacgcacca cgtgacggag
      421 cgtgaccgcg cgccgagcgc gcgccaaggt cgggcaggaa gagggcctat ttcccatgat
      481 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
      541 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      601 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
      661 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg accgtaacta
      721 ttcggtgcgt tgggcagaag cttgtgctca acgcaccgaa tagttgcggt cttttttgaa
      781 ggngggcgcg ccgaccagct ttcttg
//
