LOCUS       sequence-286872-        2561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3443
FEATURES             Location/Qualifiers
     source          1..2561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2393..2417)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2425..2466
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgagcgatca gaagaaggag
       61 gaggaggagg aggcggcagc ggcagcggcg atggctacag aaggagggaa aacctctgag
      121 ccagagaata acaataaaaa acccaaaacc tcaggctccc aggactctca gccctctcct
      181 ctggctttac tggcagctac ttgcagcaaa atagggactc ctggtgaaaa tcaagcaact
      241 ggacaacaac aaattattat agatccaagt caaggattgg tgcaacttca aaatcaacca
      301 caacagctag aactggtaac aacgcaactt gctggaaacg cttggcaact tgttgcctcc
      361 actcctcctg cttcaaaaga gaataacgtt tctcaaccag cctctagttc gtctagttct
      421 tccagcagta ataacgggag tgcatctcct acaaaaacta aatcaggtaa ttcttccacc
      481 cctggtcaat ttcaagtcat acaagtacaa aatccaagtg gtagtgtaca gtaccaagta
      541 attccacaac ttcagacagt ggaaggtcaa caaattcaaa tcaatccaac tagtagttca
      601 tctctacagg atttgcaggg tcaaattcag ctcatttctg caggtaataa tcaagctata
      661 ctcacagctg ctaacaggac agcttctggg aatattcttg ctcaaaacct ggcaaatcag
      721 acagttccgg tccaaattag acctggtgtt tcaataccac tgcagttaca gactcttcct
      781 ggtactcagg ctcaagttgt aacaacccta ccaattaaca ttggaggagt gactctagct
      841 ttgccagtga taaacaacgt ggctgccgga ggagggactg ggcaggttgg ccagcctgct
      901 gctactgctg atagtgggac ttccaatggg aatcaattag tttccacacc caccaacacc
      961 actacttctg ccagtactat gccagaatct ccctcctcct ccactacctg cacaaccact
     1021 gcttcaacgt ctttgacaag cagtgacaca ttagtgagct cagcagatac tggccagtat
     1081 gcaagcacat cagccagtag ttctgaacgc accattgaag aatctcaaac acctgctgct
     1141 actgagtctg aagcccagag ctccagtcag cttcagccta atggaatgca gaatgcacag
     1201 gatcaatcaa attctcttca gcaggtgcaa attgtaggcc aacctatctt acagcagatc
     1261 cagatccaac agcctcagca acagatcatt caggctattc caccacagtc gtttcaactc
     1321 cagtcagggc agacgattca gaccatccag cagcagcctt tacagaatgt tcaacttcaa
     1381 gcagtaaatc cgactcaggt gcttatcagg gctccaactt taacaccttc agggcaaatc
     1441 agttggcaaa ctgtacaggt tcagaatatt cagagtcttt caaatttgca agttcagaat
     1501 gctgggttat cccaacaatt aaccatcacc ccagtgtctt caagtggtgg cacaactctt
     1561 gctcagattg ctcctgtggc tgttgctggt gccccaataa ctttgaatac tgcccagctt
     1621 gcatcagtgc ctaaccttca gacagtgagc gttgccaacc tgggtgctgc aggtgttcaa
     1681 gtgcagggag ttcccgttac aatcactagt gttgcaggtc agcagcaagg acaagatgga
     1741 gtaaaagtcc agcaagctac tatagctcct gtaactgtag cagttggagg aattgctaat
     1801 gccacgatag gtgctgttag tcctgaccaa ctcacacaag tgcatttgca gcaaggccag
     1861 cagacttctg atcaagaggt acaacctggc aagaggcttc gaagagttgc ctgttcctgt
     1921 cctaattgta gggaaggaga aggaagaggc agtaatgaac caggaaaaaa gaagcagcat
     1981 atctgtcata ttgaaggatg tggtaaagtt tatggcaaaa catctcattt acgagcacat
     2041 cttcgctggc atactggaga aagacctttt atatgcaact ggatgttttg tggcaaaaga
     2101 ttcacacgga gtgatgagct ccagagacat agaagaaccc atacaggtga aaagagattt
     2161 gaatgcccgg aatgttctaa aaggtttatg cggagtgatc atctctccaa acatgtcaaa
     2221 acgcaccaga ataaaaaagg tggtgggaca gctcttgcca ttgttacctc gggagaactg
     2281 gactcatctg ttacagaggt gcttggctcc ccaagaattg tcacagttgc agccatttct
     2341 caagattcga atccagcaac tcccaatgtt tcaaccaaca tggaagaatt cttgccaact
     2401 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     2461 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2521 tcttgtggaa aggacgacaa aatcacacaa tagctgtaac t
//
