LOCUS       sequence-286878-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3445
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(647..671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             679..720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggctactct gatctatgtt
       61 gataaggaaa atggagaacc aggcacccgt gtggttgcta aggatgggct gaagctgggg
      121 tctggacctt caatcaaagc cttagatggg agatctcaag tttcaacacc acgttttggc
      181 aaaacgttcg atgccccacc agccttacct aaagctacta gaaaggcttt gggaactgtc
      241 aacagagcta cagaaaagtc tgtaaagacc aagggacccc tcaaacaaaa acagccaagc
      301 ttttctgcca aaaagatgac tgagaagact gttaaagcaa aaagctctgt tcctgcctca
      361 gatgatgcct atccagaaat agaaaaattc tttcccttca atcctctaga ctttgagagt
      421 tttgacctgc ctgaagagca ccagattgcg cacctcccct tgagtggagt gcctctcatg
      481 atccttgacg aggagagaga gcttgaaaag ctgtttcagc tgggcccccc ttcacctgtg
      541 aagatgccct ctccaccatg ggaatccaat ctgttgcagt ctccttcaag cattctgtcg
      601 accctggatg ttgaattgcc acctgtttgc tgtgacatag atattttgcc aactttcttg
      661 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
      721 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      781 ggaaaggacg agtaattagt tacctcccga gtgac
//
