LOCUS       sequence-286881-        2555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3446
FEATURES             Location/Qualifiers
     source          1..2555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2387..2411)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2419..2460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggagcc gcctcggcta
       61 cctctgactt ttgaggatgt tgctatttat ttctccgagc aagaatggca ggatctagag
      121 gcatggcaga aggaacttta caagcatgtg atgagaagca attatgagac tctcgtctct
      181 ctagatgatg gacttccaaa accagaacta atatcctgga ttgaacacgg gggagagccc
      241 ttcaggaaat ggagagaatc acagaaatca ggaaacataa tttgctcctc tgttgatatg
      301 cattttgatc caggttttga ggaacagctg ttttggggaa gccagcaggc tatgaattca
      361 ggaaaaacta aaagccattt ccaattagat cctgaaagcc agtgttcctt tggatccttt
      421 gtttccttca ggcctgacca aggcatcacc ctcgggagcc cacagagaca cgacgccagg
      481 gctcctccac cactagcctg cggccccagt gaatctaccc taaaagaagg aatcccaggt
      541 cccagaaatc tggatcttcc tggtttgtgg gacgtccccg cctgggagag cacccagcac
      601 ccttggcctg tctgcgggga aagctgttgg gagaacaacc atttagtaat gcaccagaga
      661 ggccactcaa aggaccggac acgtagggcc tgggagaaat tcaacaagag ggcggagacg
      721 cagatgccgt ggagcagccc tcgggtacag aggcacttcc ggtgtggcgt gtgcggtaag
      781 agcttccgcc ggaagctgtg tctgctgcgc catctggcgg cccacacggg gaggggcccc
      841 ttccggaacg ctgacggtga aatgtgcttc cgacacgagc tgacccatcc cagccaccgc
      901 ctcccgcagc agggggagaa gcctgcccag tgcaccccat gcggcaagcg ctccctccca
      961 gtggacagca cgcaggctcg ccggtgccag cacagccggg aggggccggc ctcttggaga
     1021 gaaggccgcg gggcctccag cagtgtgcac tcgggacaga aaccaggctc gcgcctgccc
     1081 caggagggga acagccacca ggaaggggac acggaggcgc tgcagcatgg cgcagagggg
     1141 ccctgctcct gctcggagtg tggcgagcgc tcccctatga gcgccaggct cgccagcccc
     1201 tgcagggcgc atactggaga aaagcccttc cagtgtgcgc attgcaccaa gcgcttccgc
     1261 ctgcgccgcc tgctgcaggt ccaccagcac gcgcacggtg gggagagacc gttctcctgc
     1321 aggaagtgtg gcaagggctt cgccaagcag tgtaaactca cggagcacat tcgagtccac
     1381 agcggagaga agcctttccg gtgtgccaag tgtggcagga acttccgtca gaggggacag
     1441 ctgctgcggc accagcggct gcacacggac gagaagccct ttcagtgccc agagtgtggg
     1501 ctgagcttcc gcctggagag catgctgaga gcccaccggc tccggcacgg tggggagagg
     1561 ccgttctcct gtagcgagtg tggcagaggc ttcacccacc agtgcaagct ccgtgagcac
     1621 ctgagagtgc acagcgggga gaggcccttc cagtgcctga agtgcgacaa gcgcttccgc
     1681 ctgaagggca tcctgaaggc ccaccagcac acgcacagca aggagaggcc gttctcgtgc
     1741 ggggagtgtg gcaagggctt caccagacaa tccaagctca cggagcactt gcgcgtgcac
     1801 agcggggaga gacccttcca gtgcccagag tgcaacagga gcttccgcct gaaggggcag
     1861 ctgctcagcc atcagcgcct gcacacggga gagaggccct tccagtgtcc ggagtgcgac
     1921 aagcgctatc gcgtgaaggc cgacatgaag gcccaccagc tgctgcacag cggggagatg
     1981 cctttctcct gtgagtgcgg caagggcttt gtgaaacact caaagctcat cgagcacatc
     2041 agaacgcaca cgggagagaa gccttttcag tgtcccaagt gtgacaagag tttccgcctg
     2101 aaggcgcagc tgctcagcca tcagggcctg cacacagggg agaggccttt tcactgccct
     2161 gagtgtgaca agaacttccg ggaaagggga cacatgctga ggcaccagcg catccacagg
     2221 cccgagaggc cctttgcctg tggcgattgt gggaagggct tcatttacaa gtctaagctt
     2281 gcggagcaca tcagagtaca cacaaaatcc tgtcctgctc caaatgaact ggacattaag
     2341 aaaaggctca gccaactgtt tgcaatgata gaggccgatt ggagtttgcc aactttcttg
     2401 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2461 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2521 ggaaaggacg attagtgcat ctcgaataat aacgt
//
