LOCUS       sequence-286887-        2246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3448
FEATURES             Location/Qualifiers
     source          1..2246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2078..2102)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2110..2151
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga attttgaaac ttcacggtgt
       61 gccaccctac agtactgccc tgacccttac atccagcgtt tcgtagaaac cccagctcat
      121 ttctcttgga aagaaagtta ttaccgatcc accatgtccc agagcacaca gacaaatgaa
      181 ttcctcagtc cagaggtttt ccagcatatc tgggattttc tggaacagcc tatatgttca
      241 gttcagccca ttgacttgaa ctttgtggat gaaccatcag aagatggtgc gacaaacaag
      301 attgagatta gcatggactg tatccgcatg caggactcgg acctgagtga ccccatgtgg
      361 ccacagtaca cgaacctggg gctcctgaac agcatggacc agcagattca gaacggctcc
      421 tcgtccacca gtccctataa cacagaccac gcgcagaaca gcgtcacggc gccctcgccc
      481 tacgcacagc ccagctccac cttcgatgct ctctctccat cacccgccat cccctccaac
      541 accgactacc caggcccgca cagtttcgac gtgtccttcc agcagtcgag caccgccaag
      601 tcggccacct ggacgtattc cactgaactg aagaaactct actgccaaat tgcaaagaca
      661 tgccccatcc agatcaaggt gatgacccca cctcctcagg gagctgttat ccgcgccatg
      721 cctgtctaca aaaaagctga gcacgtcacg gaggtggtga agcggtgccc caaccatgag
      781 ctgagccgtg aattcaacga gggacagatt gcccctccta gtcatttgat tcgagtagag
      841 gggaacagcc atgcccagta tgtagaagat cccatcacag gaagacagag tgtgctggta
      901 ccttatgagc caccccaggt tggcactgaa ttcacgacag tcttgtacaa tttcatgtgt
      961 aacagcagtt gtgttggagg gatgaaccgc cgtccaattt taatcattgt tactctggaa
     1021 accagagatg ggcaagtcct gggccgacgc tgctttgagg cccggatctg tgcttgccca
     1081 ggaagagaca ggaaggcgga tgaagatagc atcagaaagc agcaagtttc ggacagtaca
     1141 aagaacggtg atggtacgaa gcgcccgttt cgtcagaaca cacatggtat ccagatgaca
     1201 tccatcaaga aacgaagatc cccagatgat gaactgttat acttaccagt gaggggccgt
     1261 gagacttatg aaatgctgtt gaagatcaaa gagtccctgg aactcatgca gtaccttcct
     1321 cagcacacaa ttgaaacgta caggcaacag caacagcagc agcaccagca cttacttcag
     1381 aaacagacct caatacagtc tccatcttca tatggtaaca gctccccacc tctgaacaaa
     1441 atgaacagca tgaacaagct gccttctgtg agccagctta tcaaccctca gcagcgcaac
     1501 gccctcactc ctacaaccat tcctgatggc atgggagcca acattcccat gatgggcacc
     1561 cacatgccaa tggctggaga catgaatgga ctcagcccca cccaggcact ccctccccca
     1621 ctctccatgc catccacctc ccactgcaca cccccacctc cgtatcccac agattgcagc
     1681 attgtcagtt tcttagcgag gttgggctgt tcatcatgtc tggactattt cacgacccag
     1741 gggctgacca ccatctatca gattgagcat tactccatgg atgatctggc aagtctgaaa
     1801 atccctgagc aatttcgaca tgcgatctgg aagggcatcc tggaccaccg gcagctccac
     1861 gaattctcct ccccttctca tctcctgcgg accccaagca gtgcctctac agtcagtgtg
     1921 ggctccagtg agacccgggg tgagcgtgtt attgatgctg tgcgattcac cctccgccag
     1981 accatctctt tcccaccccg agatgagtgg aatgacttca actttgacat ggatgctcgc
     2041 cgcaataagc aacagcgcat caaagaggag ggggagtgcc caactttctt gtacaaagtg
     2101 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     2161 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2221 gagtgctgaa caccgtcgat atccgg
//
