LOCUS       sequence-286890-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3449
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1157..1181)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1230
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ccaacgacga ggcattcagc
       61 aagccctcta caccgtcgga agcccctcac gcccccgggg taccgccgca gggcagagcc
      121 gggggctttg gcaaagcgtc tggggcgcta gtgggggcgg ccagcggctc gagcgccggg
      181 ggcagcagca gaggaggcgg ctccggctcc ggggcgtcgg acctgggtgc cgggagcaag
      241 aagtccccgc ggctgcccaa gtgcgcacgc tgcaggaacc acggctacgc ctcgccgctc
      301 aagggccaca agcgcttctg catgtggcgc gactgccagt gcaagaagtg caacctgatc
      361 gccgagaggc agcgcgtgat ggccgcgcag gtggccctga gaaggcagca ggcccaggag
      421 gaggaattgg gtatcagcca ccccatccca ctgcccagtg cggccgagct gcttgtcaaa
      481 agagagaaca atggcagtaa cccgtgcctc atgactgagt gcagtggcac ctctcagcca
      541 ccgccggcca gtgtccccac cactgcagct tcagagggac gtatggtcat ccaggatatt
      601 cctgctgtca ccagcagagg gcatgtggag aacacacctg acctggtttc agactccacc
      661 tactacagca gcttctacca gccgtctctg tttccttatt acaacaatct atacaactgc
      721 ccgcagtact ccatggcctt ggctgctgat tctgcttctg gggaggtggg aaatcccctc
      781 gggggatccc ctgtgaagaa cagccttcgg ggcctccccg gaccttatgt gcctggtcag
      841 acaggaaacc agtggcagat gaagaacatg gagaaccgcc atgcaatgag ctcccagtac
      901 aggatgcatt cttactaccc gcctccctct tacctgggcc agagcgtgcc ccagttcttc
      961 acttttgagg atgctccctc ttacccggaa gccagggcga gcgtattctc gccgcccagc
     1021 agtcaagatt ctggcttggt ttccctctcg agcagctctc ctattagtaa caagagcaca
     1081 aaggcagtgc ttgaatgtga gcctgcgtcg gagcccagca gcttcacagt cactcccgtc
     1141 atcgaggagg acgagtgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1201 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1261 atttcgattt cttggcttta tatatcttgt ggaaaggacg actgcccgat ttttgccaaa
     1321 caagc
//
