LOCUS       sequence-286893-        1490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3450
FEATURES             Location/Qualifiers
     source          1..1490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1322..1346)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1354..1395
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acccatctgt gacgctgtgg
       61 cagtttctgc tgcagctgct gagagagcaa ggcaatggcc acatcatctc ctggacttca
      121 cgggatggtg gtgaattcaa gctggtggat gcagaggagg tggcccggct gtgggggcta
      181 cgcaagaaca agaccaacat gaattacgac aagctcagcc gggccttgcg gtactactat
      241 gacaagaaca tcatccgcaa ggtgagcggc cagaagttcg tctacaagtt tgtgtcctac
      301 cctgaggtcg cagggtgctc cactgaggac tgcccgcccc agccagaggt gtctgttacc
      361 tccaccatgc caaatgtggc ccctgctgct atacatgccg ccccagggga cactgtctct
      421 ggaaagccag gcacacccaa gggtgcagga atggcaggcc caggcggttt ggcacgcagc
      481 agccggaacg agtacatgcg ctcgggcctc tattccacct tcaccatcca gtctctgcag
      541 ccgcagccac cccctcatcc tcggcctgct gtggtgctcc ccagtgcagc tcctgcaggg
      601 gcagcagcgc ccccctcggg gagcaggagc accagtccaa gccccttgga ggcctgtctg
      661 gaggctgaag aggccggctt gcctctgcag gtcatcctga ccccgcccga ggccccaaac
      721 ctgaaatcgg aagagcttaa tgtggagccg ggtttgggcc gggctttgcc cccagaagtg
      781 aaagtagaag ggcccaagga agagttggaa gttgcggggg agagagggtt tgtgccagaa
      841 accaccaagg ccgagccaga agtccctcca caggagggcg tgccagcccg gctgcccgcg
      901 gttgttatgg acaccgcagg gcaggcgggc ggccatgcgg cttccagccc tgagatctcc
      961 cagccgcaga agggccggaa gccccgggac ctagagcttc cactcagccc gagcctgcta
     1021 ggtgggccgg gacccgaacg gaccccagga tcgggaagtg gctccggcct ccaggctccg
     1081 gggccggcgc tgaccccatc cctgcttcct acgcatacat tgaccccggt gctgctgaca
     1141 cccagctcgc tgcctcctag cattcacttc tggagcaccc tgagtcccat tgcgccccgt
     1201 agcccggcca agctctcctt ccagtttcca tccagtggca gcgcccaggt gcacatccct
     1261 tctatcagcg tggatggcct ctcgaccccc gtggtgctct ccccagggcc ccagaagcca
     1321 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1381 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1441 ctttatatat cttgtggaaa ggacgaacat acctataact gctacctcat
//
