LOCUS       sequence-286896-        2441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3451
FEATURES             Location/Qualifiers
     source          1..2441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2273..2297)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2305..2346
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcagaagga aatgaagatg
       61 atcaaagacg aggatgtgca tttcgacttg gctgtgaaga agaccccctc ctttccccac
      121 tgcctgcagc cagtggcttc tcggggaaag gctccccaaa gacacccctt cccggaagct
      181 ctccgagggc cattttccca gtttcggtat gaacctcccc caggagacct agatgggttc
      241 cccggggtct tcgaaggagc agggtctagg aaacggaaga gcatgcccac aaagatgccc
      301 tataaccacc ctgcagaaga agtcaccctc gccctccact cagaggagaa caaaaaccac
      361 ggccttccca acctcccttt gctgttcccg cagcccccgc gccccaagta tgactctcag
      421 atgatcgacc tgtgcaacgt gggcttccaa ttctaccgca gcctggaaca ctttgggggc
      481 aagcccgtca agcaggaacc cattaagccc agcgccgtgt ggccccagcc aacgcccact
      541 ccattcctgc ccacgcccta cccctactac cccaaagtcc acccgggcct catgttcccc
      601 ttcttcgtgc cctcgtcctc gcccttcccc ttcagccggc acaccttcct gcccaagcag
      661 cccccggaac ctctgctgcc ccggaaagcc gagccccagg agagcgagga gaccaagcag
      721 aaggtggaga gggtggacgt gaacgtgcag atcgatgaca gctactacgt ggacgtgggc
      781 ggctcgcaga agcgctggca gtgccccacc tgcgagaagt cctacacctc caagtacaac
      841 ctggtcaccc acatcctggg ccacagtggg atcaagccgc acgcgtgcac gcactgcggg
      901 aagctcttca agcagctcag ccacctgcat acccacatgc tgacccacca gggcacgcgg
      961 ccccacaagt gccaggtgtg ccacaaggcc ttcacccaga ccagccacct gaagcgccac
     1021 atgatgcagc acagcgaggt gaagccgcac aactgccgcg tgtgcggccg cggctttgcc
     1081 taccccagcg agctcaaggc ccacgaagcc aagcacgcca gtgggcgcga gaacatctgt
     1141 gtggagtgcg gcctcgactt ccccaccttg gcccagctga agagacacct caccacgcac
     1201 cggggcccca tccagtacaa ctgctccgag tgcgacaaga ccttccagta cccgagccag
     1261 ctgcagaacc acatgatgaa gcacaaggac atccggccct acatctgctc agagtgtggc
     1321 atggagtttg tgcagccgca ccacctcaag cagcactccc tcacccacaa gggtgtgaag
     1381 gagcataagt gtgggatttg tgggcgggag ttcaccctgc tggccaacat gaagcgacac
     1441 gtgctgatcc acaccaacat ccgcgcctac cagtgtcacc tctgctacaa gagcttcgtg
     1501 cagaagcaga ccctcaaggc acacatgatc gtccactctg acgtgaagcc tttcaaatgc
     1561 aagctttgtg ggaaggaatt caaccggatg cacaacctga tgggccacat gcacctgcac
     1621 tcagacagca aacccttcaa gtgcctctat tgcccaagca aattcaccct gaaggggaac
     1681 ctgacacgcc acatgaaagt caagcatgga gtcatggagc ggggccttca ttcccaaggt
     1741 ctgggaaggg ggagaatcgc cctggcacag acagccggtg tcctgaggag tctggagcag
     1801 gaggagccct ttgacctctc tcagaagcgc cgggccaagg tgccggtgtt ccagtcagac
     1861 ggggagagtg cccagggcag ccactgccac gaggaggaag aggaggataa ctgctacgag
     1921 gtggagccct acagccctgg cctggccccc cagagccagc agctctgcac acccgaggat
     1981 ctgtccacca agtcggagca cgcccccgag gtgctggagg aagcctgcaa ggaggagaag
     2041 gaggatgcat ccaagggaga atgggagaag aggagcaagg gtgaccttgg ggcagagggc
     2101 ggccaggaga gagactgtgc cggcagagat gagtgtctca gtctcagggc ttttcagagt
     2161 acccggcggg gcccctcttt ttctgattac ttatacttca agcacagaga tgagagtttg
     2221 aaagaattac tggagaggaa aatggaaaaa caagcagtgc ttttaggtat cttgccaact
     2281 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     2341 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2401 tcttgtggaa aggacgactg tcgataagcg cggcatgaac c
//
