LOCUS       sequence-28690-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Blast/shp16 (w112-1)
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    99..687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(152..172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(570..581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg
       61 aattctgcag atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
      121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
      421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
      481 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
      541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
      601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
      661 ggatctccct ttgggccgcc tccccgcctg gantctgcag atatccggtt agtaatgagt
      721 ttggaataan tctgtgg
//
