LOCUS       sequence-286902-        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3453
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1289..1313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1321..1362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gccggcgccg ggcgccagag
       61 ctgtaccggg ctccgttccc gttgtacgcg cttcaggtcg accccagcac tgggctgctc
      121 atcgctgcgg gcggaggagg cgccgccaag acaggcataa agaatggcgt gcactttctg
      181 cagctagagc tgattaatgg gcgcttgagt gcctccttgc tgcactccca tgacacagag
      241 acacgggcca ccatgaactt ggcactggct ggtgacatcc ttgctgcagg gcaggatgcc
      301 cactgtcagc tcctgcgctt ccaggcacat caacagcagg gcaacaaggc agagaaggcc
      361 ggttccaagg agcaggggcc tcgacaaagg aagggagcag ccccagcaga gaagaaatgt
      421 ggagcggaaa cccagcacga ggggctagaa ctcagggtag agaatttgca ggcggtgcag
      481 acagacttta gctccgatcc actgcagaaa gttgtgtgct tcaaccacga taataccctg
      541 cttgccactg gaggaacaga tggctacgtc cgtgtctgga aggtgcccag cctggagaag
      601 gttctggagt tcaaagccca cgaaggggag attgaagacc tggctttagg gcctgatggc
      661 aagttggtaa ccgtgggccg ggaccttaag gcctctgtgt ggcagaagga tcagctggtg
      721 acacagctgc actggcaaga aaatggaccc accttttcca gcacacctta ccgctaccag
      781 gcctgcaggt ttgggcaggt tccagaccag cctgctggcc tgcgactctt cacagtgcaa
      841 attccccaca agcgcctgcg ccagccccct ccctgctacc tcacagcctg ggatggctcc
      901 aacttcttgc cccttcggac caagtcctgt ggccatgaag tcgtctcctg cctcgatgtc
      961 agtgaatccg gcaccttcct aggcctgggc acagtcactg gctctgttgc catctacata
     1021 gctttctctc tccagtgcct ctactacgtg agggaggccc atggcattgt ggtgacggat
     1081 gtggcctttc tacctgagaa gggtcgtggt ccagagctcc ttgggtccca tgaaactgcc
     1141 ctgttctctg tggctgtgga cagtcgttgc cagctgcatc tgttgccctc acggcggagt
     1201 gttcctgtgt ggctcctgct cctgctgtgt gtcgggctta ttattgtgac catcctgctg
     1261 ctccagagtg cctttccagg tttcctttac ccaactttct tgtacaaagt ggttgatatc
     1321 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacttgcca
     1441 ccagacgttg aatcatc
//
