LOCUS       sequence-286908-        1829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3455
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1661..1685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1693..1734
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cccgaaggaa gcaaagccac
       61 ccgcagcccg tgaaatgcga gggggtcaaa gtggatactg aagactccct cgacgaagga
      121 cccggggccc tggtattgga gagtgatttg ctactaggcc aggatctgga gtttgaggag
      181 gaagaggaag aggaggaagg cgacggcaac agtgaccagc tcatgggctt cgagagagac
      241 tcggaaggag actctctggg ggccaggcct gggcttccct atgggctgag cgacgatgag
      301 tctgggggcg gccgggcact aagtgcggag agtgaagttg aggagccagc caggggtcca
      361 ggggaggcca ggggtgagag gccaggccca gcctgccagc tgtgtggggg gccgacaggt
      421 gaggggccgt gttgtggggc aggagggccg ggtggggggc ccctgctgcc cccacggcta
      481 ctgtactcat gccgcctctg caccttcgtg tcccactact cgagccacct gaagcggcac
      541 atgcagacac acagcggaga gaagccgttc cgctgtggcc gctgccccta cgcctcagcc
      601 cagctcgtca acctgacacg acatacccgc acccacactg gcgagaagcc ctaccgctgt
      661 ccccactgcc cctttgcctg cagcagcctg ggcaacctga ggcggcatca gcgtacccac
      721 gcagggcccc ccactcctcc ctgcccgacc tgtggcttcc gctgctgtac tccacgacca
      781 gcccggcctc ccagtcccac agagcaggag ggggcggtgc cccggcgacc tgaagatgct
      841 ctgctccttc cagatttgag cctccatgtg ccaccaggtg gtgccagttt cctgccagac
      901 tgtgggcagc tgcggggtga aggggagggc ctctgcggga ctggatcaga accactgcca
      961 gagctgctat tcccttggac ctgccggggc tgtggacaag agctggagga gggtgagggt
     1021 agtcggctgg gagctgccat gtgtgggcgc tgcatgcgag gagaggctgg agggggtgcc
     1081 agtggggggc cccagggccc cagtgacaaa ggctttgcct gtagcctctg cccctttgcc
     1141 actcactatc ccaaccacct ggcccggcac atgaagacac acagtggtga gaagcccttc
     1201 cgctgcgccc gctgtcctta tgcctctgct catctggata acctgaaacg gcaccagcgc
     1261 gtccatacag gagagaagcc ctacaagtgc cccctctgcc cttatgcctg tggcaatctg
     1321 gccaacctca agcgtcatgg tcgcatccac tctggtgaca aaccttttcg gtgtagcctt
     1381 tgcaactaca gctgcaacca gagcatgaac ctcaaacgtc acatgctgcg gcacacaggc
     1441 gagaagccct tccgctgtgc cacctgcgcc tataccacgg gccactggga caactacaag
     1501 cgccaccaga aggtgcatgg ccacggtggg gcaggagggc ctggtctctc tgcctctgag
     1561 ggctgggccc cacctcatag cccaccctct gttttgagct ctcggggccc accagccctg
     1621 gggactgctg gcagccgggc tgtccacaca gactcatcct gcccaacttt cttgtacaaa
     1681 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1741 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1801 gacgactgtt ctcagcttga acagatatt
//
