LOCUS       sequence-28691-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV/TO RasV12 Puro (w119-1)
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    35..53
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(193..225,226..228,229..762)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 attagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
       61 ctagtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggcttt
      121 aaaggaacca attcagtcga ctggatccca gtgtggtggt acagcttggg gggccagggc
      181 cggggccgag cgatgacgga atataagctg gtggtggtgg gcgccgtcgg tgtgggcaag
      241 agtgcgctga ccatccagct gatccagaac cattttgtgg acgaatacga ccccactata
      301 gaggattcct accggaagca ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg
      361 gataccgccg gccaggagga gtacagcgcc atgcgggacc agtacatgcg caccggggag
      421 ggcttcctgt gtgtgtttgc catcaacaac accaagtctt ttgaggacat ccaccagtac
      481 agggagcaga tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct ggtggggaac
      541 aagtgtgacc tggctgcacg cactgtggaa tctcggcagg ctcaggacct cgcccgaagc
      601 tacggcatcc cctacatcga gacctcggcc aagacccggc agggagtgga ggatgccttc
      661 tacacgttgg tgcgtgagat ccggcagcac aagctgcgga agctgaaccc tcctgatgag
      721 agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gacgcagcac agctcaggac
      781 atg
//
