LOCUS       sequence-286911-        1868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3456
FEATURES             Location/Qualifiers
     source          1..1868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1700..1724)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1732..1773
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagctgctga tttgtcccat
       61 ggacattatc tttctgggga cccagtttgc cttcatgaag aaaagacacc agcaggaaga
      121 atagtggctg actgcctaac agattgttat caggattcag tgacctttga cgatgtggct
      181 gtggacttca cccaggagga gtggacttta ctggactcaa ctcagagaag cctctacagt
      241 gacgtgatgc tggagaacta caagaacctg gccacagtag gaggtcagat catcaaaccc
      301 agtctaatct cttggttgga acaagaagag tcaaggacag ttcagggagg agttctccaa
      361 ggatgggaaa tgcgacttga aacccagtgg tctatacttc agcaggactt tttgaggggt
      421 cagacatcca ttgggataca attggaagga aaacacaatg gaagggaact ctgtgactgt
      481 gagcaatgtg gagaagtctt cagtgaacac tcatgcctta agacgcacgt gagaactcaa
      541 agtacaggga acactcatga ctgtaatcag tatggaaaag atttccttac cctgtgtgag
      601 aaaacctcta ctggtgagaa actttctgag tttaatcaga gtgaaaaaat cttcagcctg
      661 acaccaaata ttgtatacca gagaactagc acacaagaaa agtcatttga atgtagtcac
      721 tgtggaaaat ccttcattaa tgagtcatac cttcaggcac atatgagaac tcacaatgga
      781 gaaaaactct acgaatggag gaattatggg ccaggtttta ttgactctac aagcctttct
      841 gtgcttatag aaaccctcaa tgcaaaaaag ccctacaaat gtaaggaatg tggaaaaggc
      901 tatagatacc cagcctacct cagtattcac atgcgaaccc acactgggga gaaaccatat
      961 gaatgtaagg aatgtgggaa agccttcaat tattccaact catttcagat acatggaaga
     1021 actcacactg gagagaaacc ctatgtatgt aaggaatgtg ggaaagcctt cactcagtac
     1081 tcgggcctta gtatgcatgt acgatctcac agtggagaca agccctatga atgtaaggaa
     1141 tgtgggaaat ccttccttac atcctcacgc cttattcaac atataagaac tcacactgga
     1201 gagaagcctt ttgtatgtgt tgaatgtggg aaagcctttg cagtttcctc aaatcttagt
     1261 ggacatttga gaactcacac tgaagagaag gcctgtgagt gtaagatatg tgggaaagta
     1321 tttgggtatc cctcatgtct taataatcac atgcgaacgc acagtgccca gaaaccatac
     1381 acctgtaagg aatgtgggaa ggcttttaac tattccaccc accttaaaat tcacatgcga
     1441 atccacactg gagaaaaacc ctatgagtgt aaacaatgtg gaaaggcctt cagtcattcc
     1501 agttcatttc aaatacatga aaggactcac actggagaga aaccctatga atgcaaggag
     1561 tgtgggaaag ccttcacgtg ttccagttcc tttagaattc atgaaaaaac tcacacagaa
     1621 gagaaaccct ataaatgtca gcaatgcggg aaagcttaca gtcatccccg ttcacttcga
     1681 agacatgaac aaattcacta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1741 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgcaaa cattctatca
     1861 gcggacca
//
