LOCUS       sequence-286914-        1374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3457
FEATURES             Location/Qualifiers
     source          1..1374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             318..326
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(1217..1241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1249..1290
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg catcaggcag ggcacgctgc
       61 acccgaaaac tccggaactg ggtggtggag caagtggaga gtgggcagtt tcccggagtg
      121 tgctgggatg atacagctaa gaccatgttc cggattccct ggaaacatgc aggcaagcag
      181 gacttccggg aggaccagga tgctgccttc ttcaaggcct gggcaatatt taagggaaag
      241 tataaggagg gggacacagg aggtccagct gtctggaaga ctcgcctgcg ctgtgcactc
      301 aacaagagtt ctgaatttaa ggaggttcct gagaggggcc gcatggatgt tgctgagccc
      361 tacaaggtgt atcagttgct gccaccagga atcgtctctg gccagccagg gactcagaaa
      421 gtaccatcaa agcgacagca cagttctgtg tcctctgaga ggaaggagga agaggatgcc
      481 atgcagaact gcacactcag tccctctgtg ctccaggact ccctcaataa tgaggaggag
      541 ggggccagtg ggggagcagt ccattcagac attgggagca gcagcagcag cagcagccct
      601 gagccacagg aagttacaga cacaactgag gccccctttc aaggggatca gaggtccctg
      661 gagtttctgc ttcctccaga gccagactac tcactgctgc tcaccttcat ctacaacggg
      721 cgcgtggtgg gcgaggccca ggtgcaaagc ctggattgcc gccttgtggc tgagccctca
      781 ggctctgaga gcagcatgga gcaggtgctg ttccccaagc ctggcccact ggagcccacg
      841 cagcgcctgc tgagccagct tgagaggggc atcctagtgg ccagcaaccc ccgaggcctc
      901 ttcgtgcagc gcctttgccc catccccatc tcctggaatg caccccaggc tccacctggg
      961 ccaggcccgc atctgctgcc cagcaacgag tgcgtggagc tcttcagaac cgcctacttc
     1021 tgcagagact tggtcaggta ctttcagggc ctgggccccc caccgaagtt ccaggtaaca
     1081 ctgaatttct gggaagagag ccatggctcc agccatactc cacagaatct tatcacagtg
     1141 aagatggagc aggcctttgc ccgatacttg ctggagcaga ctccagagca gcaggcagcc
     1201 attctgtccc tggtgtaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1261 cctaaccctc tcctcggtct cgattctacg tagtaatgaa cttgaaagta tttcgatttc
     1321 ttggctttat atatcttgtg gaaaggacga taagtccgag acgccggact gcca
//
