LOCUS       sequence-286917-        1571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3458
FEATURES             Location/Qualifiers
     source          1..1571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1403..1427)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1435..1476
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acacagtggt ctttgaagac
       61 gtggttgtgg atttcacgct ggaggagtgg gccttgctga atcctgctca gagaaaactc
      121 tacagagatg tcatgctgga gaccttcaag cacctggcct cagtagataa tgaggctcag
      181 cttaaagcca gtgggtctat ttctcagcag gatacttctg gagaaaaatt atccctcaaa
      241 cagaaaatag aaaagttcac aagaaagaat atatgggcct cccttttagg aaaaaattgg
      301 gaagaacata gcgttaaaga caagcacaac accaaggaga gacatttgag aaatccaagg
      361 gtggagagac catgtaaaag cagtaaaggt aataaacgtg gaagaacctt cagaaagact
      421 cgaaattgta atcgtcatct gcgcaagaat tgttgtacta gtgtaagacg gtacgaatgc
      481 agtcagtgtg gaaaactctt cacccattcc tcatccctga taaggcacaa aagagctcac
      541 tctggacaaa aattatataa atgtaaggaa tgtgggaaag ccttcagtcg cccttcctac
      601 ctacagacgc atgagaaaac tcacagtgga gagaaaccct atgcctgtca atcttgcggg
      661 aagacatttc ttcgttccca ctctctcact gaacatgtaa ggactcacac tggagagaaa
      721 ccctacgaat gtgggcagtg tgggaaaggc ttcagttgtc ccaaatcctt tcgcgcacat
      781 gtgatgatgc acgccggagg gagaccgtat gagtgcaagc actgtgggaa agccttcagg
      841 tgtcagaaat cctttcgagt ccatatgatc atgcacgccg gagggagacc gtatgagtgc
      901 aagcagtgtg ggaaagccta ctgctgggca acatcctttc aacgacacgt gagaattcac
      961 aacggggaga aaccctataa gtgtggaaaa tgcgggaaag cattcggttg gccctcatcc
     1021 ttacacaaac acgcgagaac gcatgctaaa aagaaacctg tgagtggggg cagcgtggga
     1081 aagtcttccg cgaggcctcg cccctccaca gatgtcaaat cacaaactag agagaaagtc
     1141 tataaatgtg aaacgtgtgg gaaaacgtat ggttggtcct catctttaca caaacatgag
     1201 agaaagcaca ctggggagaa acctgtaaat gcagccagtg tgggaaaacc ttcaggcggg
     1261 ctttgctctt ccaaaaatgt aagaacgcag attggacaga agcccagtaa atgcgaaaaa
     1321 tgtgggaaag ctttcagttg tcccaaagcc tttcaaggtc atgtgagaag tcacacagga
     1381 aagaaatcct gtacatctaa gtacccaact ttcttgtaca aagtggttga tatcggtaag
     1441 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1501 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagtt tcaggaccac
     1561 aataagccct c
//
