LOCUS       sequence-28692-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV/TO p16 Hygro (w184-1)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(647..671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(755..775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 acattagcag agctctccta tcagtgatag agatctccct atcagtgata gagatcgtcg
       61 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt
      121 taaaggaacc aattcagtcg actggatcca gcatggagcc ttcggctgac tggctggcca
      181 cggccgcggc ccggggtcgg gtagaggagg tgcgggcgct gctggaggcg ggggcgctgc
      241 ccaacgcacc gaatagttac ggtcggaggc cgatccaggt catgatgatg ggcagcgccc
      301 gagtggcgga gctgctgctg ctccacggcg cggagcccaa ctgcgccgac cccgccactc
      361 tcacccgacc cgtgcacgac gctgcccggg agggcttcct ggacacgctg gtggtgctgc
      421 accgggccgg ggcgcggctg gacgtgcgcg atgcctgggg ccgtctgccc gtggacctgg
      481 ctgaggagct gggccatcgc gatgtcgcac ggtacctgcg cgcggctgcg gggggcacca
      541 gaggcagtaa ccatgcccgc atagatgccg cggaaggtcc ctcagacatc cccgattgaa
      601 aggaattctg cagatatcca tcacactggc cgctcgagat atctagaccc agctttcttg
      661 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
      721 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
      781 acgctgcttt atgccttgta tcatgctatg cttccgtatg gtttcatttc tctcttgnna
      841 atct
//
