LOCUS       sequence-286920-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3459
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(665..689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             697..738
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gtaaaggaga ccccaacaag
       61 ccgcggggca aaatgtcctc gtacgccttc ttcgtgcaga cctgccggga agagcacaag
      121 aagaaacacc cggactcttc cgtcaatttc gcggaattct ccaagaagtg ttcggagaga
      181 tggaagacca tgtctgcaaa ggagaagtcg aagtttgaag atatggcaaa aagtgacaaa
      241 gctcgctatg acagggagat gaaaaattac gttcctccca aaggtgataa gaaggggaag
      301 aaaaaggacc ccaatgctcc taaaaggcca ccatctgcct tcttcctgtt ttgctctgaa
      361 catcgcccaa agatcaaaag tgaacaccct ggcctatcca ttggggatac tgcaaagaaa
      421 ttgggtgaaa tgtggtctga gcagtcagcc aaagataaac aaccatatga acagaaagca
      481 gctaagctaa aggagaaata tgaaaaggat attgctgcat atcgtgccaa gggcaaaagt
      541 gaagcaggaa agaagggccc tggcaggcca acaggctcaa agaagaagaa cgaaccagaa
      601 gatgaggagg aggaggagga agaagaagat gaagatgagg aggaagagga tgaagatgaa
      661 gaatacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
      721 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
      781 tggctttata tatcttgtgg aaaggacgat cgtgcccaca aaacatgtaa agt
//
