LOCUS       sequence-286923-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3460
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             793..834
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tgtcctctac agccatgtat
       61 tcggctcctg gcagagactt ggggatggaa ccgcacagag ccgcgggccc tttgcagctg
      121 cgattttcgc cctacgtttt caacggaggt actatactgg caattgctgg agaagatttt
      181 gcaattgttg cttctgatac tcgattgagt gaagggtttt caattcatac gcgggatagc
      241 cccaaatgtt acaaattaac agacaaaaca gtcattggat gcagcggttt tcatggagac
      301 tgtcttacgc tgacaaagat tattgaagca agactaaaga tgtataagca ttccaataat
      361 aaggccatga ctacgggggc aattgctgca atgctgtcta caatcctgta ttcaaggcgc
      421 ttctttccat actatgttta caacatcatc ggtggacttg atgaagaagg aaagggggct
      481 gtatacagct ttgatccagt agggtcttac cagagagact ccttcaaggc tggaggctca
      541 gcaagtgcca tgctacagcc cctgcttgac aaccaggttg gttttaagaa catgcagaat
      601 gtggagcatg ttccgctgtc cttggacaga gccatgcggc tggtgaaaga tgtcttcatt
      661 tctgcggctg agagagatgt gtacactggg gacgcactcc ggatctgcat agtgaccaaa
      721 gagggcatca gggaggaaac tgtttcctta aggaaggact gcccaacttt cttgtacaaa
      781 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      841 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      901 gacgaagtga aagaatcacc cctatgaac
//
