LOCUS       sequence-286926-        1640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3461
FEATURES             Location/Qualifiers
     source          1..1640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1472..1496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1504..1545
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggatggatt ttatgaccag
       61 caagtgcctt acatggtcac caatagtcag cgtgggagaa attgtaacga gaaaccaaca
      121 aatgtcagga aaagaaaatt cattaacaga gatctggctc atgattcaga agaactcttt
      181 caagatctaa gtcaattaca ggaaacatgg cttgcagaag ctcaggtacc tgacaatgat
      241 gagcagtttg taccagacta tcaggctgaa agtttggctt ttcatggcct gccactgaaa
      301 atcaagaaag aaccccacag tccatgttca gaaatcagct ctgcctgcag tcaagaacag
      361 ccctttaaat tcagctatgg agaaaagtgc ctgtacaatg tcagtgccta tgatcagaag
      421 ccacaagtgg gaatgaggcc ctccaacccc cccacaccat ccagcacgcc agtgtcccca
      481 ctgcatcatg catctccaaa ctcaactcat acaccgaaac ctgaccgggc cttcccagct
      541 cacctccctc catcgcagtc cataccagat agcagctacc ccatggacca cagatttcgc
      601 cgccagcttt ctgaaccctg taactccttt cctcctttgc cgacgatgcc aagggaagga
      661 cgtcctatgt accaacgcca gatgtctgag ccaaacatcc ccttcccacc acaaggcttt
      721 aagcaggagt accacgaccc agtgtatgaa cacaacacca tggttggcag tgcggccagc
      781 caaagctttc cccctcctct gatgattaaa caggaaccca gagattttgc atatgactca
      841 gaagtgccta gctgccactc catttatatg aggcaagaag gcttcctggc tcatcccagc
      901 agaacagaag gctgtatgtt tgaaaagggc cccaggcagt tttatgatga cacctgtgtt
      961 gtcccagaaa aattcgatgg agacatcaaa caagagccag gaatgtatcg ggaaggaccc
     1021 acataccaac ggcgaggatc acttcagctc tggcagtttt tggtagctct tctggatgac
     1081 ccttcaaatt ctcattttat tgcctggact ggtcgaggca tggaatttaa actgattgag
     1141 cctgaagagg tggcccgacg ttggggcatt cagaaaaaca ggccagctat gaactatgat
     1201 aaacttagcc gttcactccg ctattactat gagaaaggaa ttatgcaaaa ggtggctgga
     1261 gagagatatg tctacaagtt tgtgtgtgat ccagaagccc ttttctccat ggcctttcca
     1321 gataatcagc gtccactgct gaagacagac atggaacgtc acatcaacga ggaggacaca
     1381 gtgcctcttt ctcactttga tgagagcatg gcctacatgc cggaaggggg ctgctgcaac
     1441 ccccacccct acaacgaagg ctacgtgtat ttgccaactt tcttgtacaa agtggttgat
     1501 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1561 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacaca
     1621 ggttacagtg gtgttcagcc
//
