LOCUS       sequence-286929-        3866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3462
FEATURES             Location/Qualifiers
     source          1..3866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3698..3722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3730..3771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gggtggactt tgatgtgaag
       61 actttctgcc acaacttgcg ggcgactaag ccaccatacg agtgcccggt ggagacctgc
      121 cgaaaggtct acaagagtta cagtggtatt gagtaccacc tgtaccacta tgaccacgac
      181 aacccaccac ccccacaaca aactccactc cgcaagcaca agaaaaaggg gcgccagtca
      241 cgcccagcca acaagcagtc acccagcccc tcagaggtct cacagtcacc aggccgtgag
      301 gtgatgagct atgcacaggc ccagcgcatg gtggaggtgg acttgcatgg ccgcgtccac
      361 cgcatcagca tctttgacaa cctggatgtg gtgtcagagg atgaggaagc ccccgaggag
      421 gcccctgaga atggcagcaa caaggagaac actgagacac cagctgctac tcccaagtca
      481 ggcaaacata agaacaagga gaagcgcaag gactccaacc atcatcacca ccacaatgtt
      541 tctgcgagca ccactcccaa gctgccagag gtggtctatc gggagctgga acaggacacc
      601 cctgatgccc caccccggcc aacttcctat taccggtaca tcgagaagtc tgcagaggag
      661 ctggacgagg aagtagagta tgacatggac gaggaggact acatctggct ggatatcatg
      721 aatgagcgtc ggaagacaga gggtgtaagt cccatcccgc aggagatctt tgagtaccta
      781 atggaccgac tggagaagga gtcgtacttt gagagtcata ataaaggcga ccctaatgcg
      841 ctagtggacg aggatgctgt ttgctgtatc tgcaatgatg gtgagtgcca gaacagcaat
      901 gtcatcctct tctgtgacat gtgcaacctg gccgtgcacc aggagtgcta cggtgtcccc
      961 tatatccctg agggccagtg gctgtgccgc cgttgcctgc agtcaccctc tcgtgctgtg
     1021 gattgtgccc tgtgccccaa caagggcggt gccttcaagc agacagatga cgggcgctgg
     1081 gcccatgtgg tgtgtgcctt gtggatccct gaggtctgct tcgccaacac ggtcttccta
     1141 gagcctattg acagcattga gcacatccca ccagctcgct ggaagctcac ctgctacatt
     1201 tgcaaacaac ggggctcagg ggcctgcatc cagtgccaca aggccaactg ttacacagct
     1261 ttccatgtga catgcgccca gcaggctggc ctttacatga agatggagcc tgtgcgggag
     1321 acaggcgcca acggcacctc tttcagtgtc cgcaagacag cctactgcga catccacacg
     1381 cctccaggtt cagcacgccg actgcctgcc ctgtcccaca gcgagggtga ggaggatgaa
     1441 gatgaggagg aggatgaggg taagggctgg agctcagaga aagtcaagaa ggccaaggcc
     1501 aagtcccgga tcaaaatgaa gaaggcacgg aagatcctgg cagagaagcg ggcagcagca
     1561 cctgtggtgt cagtgccctg catcccacca cacaggctta gtaaaatcac caaccgcctg
     1621 accatccaaa ggaagagcca gttcatgcag aggctgcaca gctactggac actgaagcgg
     1681 cagtcacgga atggggtccc attgctacgt cgcctgcaga cacacctgca atctcagagg
     1741 aactgtgacc aagttgggag agattctgag gataagaact gggcccttaa agaacagctc
     1801 aagtcatggc agcggctccg gcatgacttg gagcgagctc ggctgctcgt ggaattgatc
     1861 cgcaagcggg aaaaactcaa aagggagacg atcaaggttc agcagattgc catggagatg
     1921 cagctgactc ctttcctcat cctccttcgc aaaaccttgg agcagctcca agagaaggac
     1981 acaggcaaca tcttcagcga gccggtccct ctgtctgagg taaccgaatt ggacgaagta
     2041 cctgactacc tagaccacat caaaaagccc atggactttt tcaccatgaa gcagaacttg
     2101 gaggcttacc gctacctgaa ttttgatgat tttgaggagg acttcaacct catcgtcagc
     2161 aactgcctca agtataacgc caaggacacc atcttctacc gggcagcagt gcggcttcgt
     2221 gagcagggtg gtgctgtgct ccgccaggcc cggcgccagg cagaaaaaat gggcattgac
     2281 tttgagacgg gcatgcatat cccccacagc ctggctggag atgaggccac acaccacact
     2341 gaagatgcag ccgaggaaga gcggctggtc ttgctggaga accagaagca cctgccagtg
     2401 gaagaacagc taaagctgct tctggagcgg ctggacgaag tgaatgccag caagcagagt
     2461 gtgggccgct cacggcgtgc aaagatgatc aagaaagaga tgacggcact gcggcggaag
     2521 cttgcccatc agcgagagac gggacgtgat ggccctgagc ggcatggccc ctcgagccgg
     2581 ggtagtctga caccccaccc ggcagcctgt gacaaggatg ggcagacaga tagtgcggca
     2641 gaggagagca gcagccagga gacaagcaaa ggcctgggtc ccaacatgtc ctcaaccccc
     2701 gcacatgagg tgggcaggag aacctcagtt ctgttctcca aaaagaaccc gaagacagct
     2761 ggaccgccca agaggccggg ccggcccccc aaaaaccggg agagccagat gacccccagc
     2821 cacggaggca gtcctgtggg gcccccccag ctccccatca tgagttccct gcgtcagcgc
     2881 aagcggggta ggagcccccg gcccagttcg agctcagaca gcgacagtga taagtccaca
     2941 gaagaccccc caatggactt accagccaat ggcttcagcg gtggaaacca accagtgaag
     3001 aagagtttct tggtataccg taatgactgc agccttcccc ggagcagctc agactctgag
     3061 tccagcagca gtagcagtag cagcgctgct tcagaccgga ccagcacaac gccctcaaaa
     3121 caaggccggg gcaaaccctc cttctctcgg ggcactttcc cagaggacag cagtgaggat
     3181 acctcaggca ctgagaatga ggcctactcc gtgggcactg gccgcggcgt gggccacagc
     3241 atggtaagga agagtctggg ccggggagct ggctggctgt cagaggatga ggactccccg
     3301 ctggatgctc tggacctcgt gtgggccaaa tgccgaggct atccatcata cccagctctg
     3361 atcattgatc caaagatgcc ccgagaaggt atgttccacc atggggttcc catccctgtg
     3421 cccccactgg aggtgctgaa acttggggag cagatgaccc aggaagcccg agagcatctc
     3481 tacctcgtcc tcttctttga caacaaacga acctggcagt ggctgcccag gaccaagctg
     3541 gttcctctgg gtgtgaacca ggacctagac aaggagaaga tgctggaggg ccgcaagtcc
     3601 aacatccgca agtcagtaca gatcgcctac cacagggctc tgcagcaccg cagcaaggtg
     3661 caaggcgagc agagcagtga gaccagtgat agtgattgcc caactttctt gtacaaagtg
     3721 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     3781 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3841 gactatgcaa atagttgcac ttatta
//
