LOCUS       sequence-286935-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3464
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(728..752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             760..801
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgtgttggc tagttatagt
       61 atattccagg agctacaact tgtccacgac accggctact tctcagcttt accatccctg
      121 gaggagacct ggcagcagac atgccttgaa ttggaacgct acctacagac ggagccccgg
      181 aggatctcag agacctttgg tgaggacttg gactgtttcc tccacgcttc ccctcccccg
      241 tgcattgagg aaagcttccg tcgcttagac cccctgctgc tccccgtgga agcggccatc
      301 tgtgagaaga gctcggcagt ggacatcttg ctctctcggg acaagttgct atctgagacc
      361 tgcctcagcc tccagccggc cagctcttct ctagacagct acacagccgt caaccaggcc
      421 cagctcaacg cagtgacctc attaacgccc ccatcgtccc ctgagctcag ccgccatctg
      481 gtcaaaacct cacaaactct ctctgccgtg gatggcacgg tgacgttgaa actggtggcc
      541 aagaaggctg ctctcagctc cgtaaaggtg agaagcctta taagtgctca tgggagggat
      601 gtgagtggcg ttttgcacga agcgatgagc tcacgaggca ctacaggaaa cacacaggtg
      661 caaagccctt caaatgcaac cactgcgaca ggtgtttttc caggtctgac catcttgccc
      721 tccacatgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      781 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      841 tcttggcttt atatatcttg tggaaaggac gaccacttgt tccccgaccc cataag
//
