LOCUS       sequence-286938-        2270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3465
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2102..2126)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2134..2175
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acggctcctt cgtccagcac
       61 agtgtgaggg ttctgcagga gctcaacaag cagcgggaga agggccagta ctgcgacgcc
      121 actctggacg tggggggcct ggtgtttaag gcacactgga gtgtccttgc ctgctgcagt
      181 cactttttcc agagcctcta cggggatggc tcagggggca gtgtcgtcct ccctgctggc
      241 ttcgctgaga tctttggcct cttgttggac tttttctaca ctggtcacct cgctctcacc
      301 tcagggaacc gggatcaggt gctcctggca gccagggagt tgcgagtgcc agaggccgta
      361 gagctgtgcc agagcttcaa gcccaaaact tcagtgggac aggcagcagg tggccagagt
      421 gggctggggc cccctgcctc ccagaatgtg aacagccacg tcaaggagcc ggcaggcttg
      481 gaagaagagg aagtttcgag gactctgggt ctagtcccca gggatcagga gcccagaggc
      541 agtcatagtc ctcagaggcc ccagctccat tccccagctc agagtgaggg cccctcctcc
      601 ctctgtggga aactgaagca ggccttgaag ccttgtcccc ttgaggacaa gaaacccgag
      661 gactgcaaag tgcccccaag gcccttagag gctgaaggtg cccagctgca gggcggcagt
      721 aatgagtggg aagtggtggt tcaagtggag gatgatgggg atggcgatta catgtctgag
      781 cctgaggctg tgctgaccag gaggaagtca aatgtaatcc gaaagccctg tgcagctgag
      841 ccagccctga gcgcgggctc cctagcagct gagcctgctg agaacagaaa aggtacagcg
      901 gtgccggtcg aatgccccac atgtcataaa aagttcctca gcaaatatta tctaaaagtc
      961 cacaacagga aacatactgg ggagaaaccc tttgagtgtc ccaaatgtgg gaagtgttac
     1021 tttcggaagg agaacctcct ggagcatgaa gcccggaatt gcatgaaccg ctcggaacag
     1081 gtcttcacgt gctctgtgtg ccaggagaca ttccgccgaa ggatggagct gcgggtgcac
     1141 atggtgtctc acacagggga gatgccctac aagtgttcct cctgctccca gcagttcatg
     1201 cagaagaagg acttgcagag ccacatgatc aaacttcatg gagcccccaa gccccatgca
     1261 tgccccacct gtgccaagtg cttcctgtct cggacagagc tgcagctgca tgaagctttc
     1321 aagcaccgtg gtgagaagct gtttgtgtgt gaggagtgtg ggcaccgggc ctcgagccgg
     1381 aatggcctgc agatgcacat caaggccaag cacaggaatg agaggccaca cgtatgtgag
     1441 ttctgcagcc acgccttcac ccaaaaggcc aatctcaaca tgcacctgcg cacacacacg
     1501 ggtgagaagc ccttccagtg ccacctctgt ggcaagacct tccgaaccca agccagcctg
     1561 gacaagcaca accgcaccca caccggggaa aggcccttca gttgcgagtt ctgtgaacag
     1621 cgcttcactg agaaggggcc cctcctgagg cacgtggcca gccgccatca ggagggccgg
     1681 ccccacttct gccagatatg cggcaagacc ttcaaagccg tggagcaact gcgtgtgcac
     1741 gtcagacggc acaagggggt gaggaagttt gagtgcaccg agtgtggcta caagtttacc
     1801 cgacaggccc acctgcggag gcacatggag atccacgacc gggtagagaa ctacaacccg
     1861 cggcagcgca agctccgcaa cctgatcatc gaggacgaga agatggtggt ggtggcgctg
     1921 cagccgcctg cagagctgga ggtgggctcg gcggaggtca ttgtggagtc cctggcccag
     1981 ggcggcctgg cctcccagct ccccggccag agactgtgtg cagaggagag cttcaccggc
     2041 ccaggtgtcc tggagccctc cctcatcatc acagctgctg tccccgagga ctgtgacaca
     2101 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2161 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2221 ctttatatat cttgtggaaa ggacgatgtt agcaaacgta gtaaaaagtc
//
