LOCUS       sequence-286950-        2693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3469
FEATURES             Location/Qualifiers
     source          1..2693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2059..2070
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2525..2549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2557..2598
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcagaattc tgtatcagta
       61 ccaccaaaag atgaaggtga atcaaacatc ccttctggaa caatacagag taggaaaggt
      121 ttgcagaata agagtcagtt taggaccatt gcaccaaaaa ttgtgcccaa agtcctaacg
      181 tccagaatgc tgccatgtca ttcaccatca cgctctgatc aggtgaatct gggaccctcc
      241 atcaactcca agctgctggg gatgtccacc cagaactatg ccctgatgca ggttgctggc
      301 caggagggga cattttctct tgttgctctg ccacatgttg cctcagctca gccaattcag
      361 aaacccagaa tgtccctacc tgaaaacctg aaacttccta ttcctagata tcaaccccct
      421 agaaatagca aagcatcaag aaagaaaccc atcctgatct ttcctaagag tggctgtagc
      481 aaagctcctg cccaaaccca aatgtgtcct cagatgtccc cttccccacc tcaccaccct
      541 gaactcctgt acaaacccag tccatttgag gaagtaccat cactagagca agccccagcc
      601 agcattagca cagctgcgct gaccaatgga agtgaccatg gggacttgag accaccagtg
      661 accaacaccc atggcagtct gaaccctcct gctaccccag catcatccac accagaggag
      721 cctgccaagc aggacctcac agctctttca gggaaagcac actttgtaag caagataaca
      781 tctagtaaac cttctgctgt tgccagtgaa aaatttaaag aacaagttga tcttgcaaaa
      841 accatgacca atttatcacc aaccattctt ggcaatgcag ttcagttgat ctcttcagtc
      901 cccaaaggga aactgccaat cccaccctac tcaagaatga agacaatgga ggtttacaaa
      961 atcaaatcag atgctaacat tgcaggtttt tctttaccag gacctaaggc cgactgtgat
     1021 aagataccct ccaccacaga aggctttaat gcagccacca aggtggcaag caggctacct
     1081 gttccacaag tgtcacagca gagtgcctgt gaaagtgcct tttgtccacc caccaaactt
     1141 gatcttaacc acaaaacaaa actgaacagt ggagcagcaa agagaaaagg aagaaaacgg
     1201 aaggtaccag atgaaatttt ggcatttcag ggaaaaagga ggaaatatat cattaataag
     1261 tgtagagatg gtaaagaaag agtaaaaaat gatccccaag aattcagaga ccaaaagctg
     1321 gggaccctga aaaaataccg tagcattatg cccaaaccta tcatggtcat acccactttg
     1381 gcctccctgg cttctccaac tacactacag tcccagatgc ttgggggcct aggacaggat
     1441 gttttgttaa ataattcact cactcctaaa tatcttggct gtaagcaaga caacagctct
     1501 tcccctaagc ccagctccgt gttcagaaat ggattctctg gcattaagaa gccttggcac
     1561 agatgtcacg tctgcaacca ccacttccag ttcaaacagc accttcgaga ccacatgaat
     1621 acacacacca acagacgccc ttacagttgt cggatttgtc gcaagtccta tgtacgtcct
     1681 ggcagcctga gcacacacat gaaacttcat catggtgaga accgtctgaa gaaactcatg
     1741 tgttgtgagt tttgtgcaaa agtgtttggc cacatccgag tctattttgg ccatctgaaa
     1801 gaagtgcata gggttgtgat cagcactgag cctgcgccca gtgaactgca gccaggagac
     1861 ataccaaaga acagagacat gagtgtgcga ggcatggagg gatcattgga gagggaaaac
     1921 aagtccaacc tggaagaaga cttccttcta aaccaggcag acgaagtcaa attacaaatc
     1981 aaatgtggtc gttgtcagat tactgctcag tcttttgcgg aaataaaatt tcatttactt
     2041 gatgttcatg gagaggaaat tgagggcagg ctacaagaag ggaccttccc aggaagcaag
     2101 gggactcagg aagagttggt gcagcacgct agccccgact ggaaaaggca tcctgagaga
     2161 gggaagccgg agaaggttca ttcctcctcc gaggaatcac atgcatgtcc aagactgaaa
     2221 aggcagctcc accttcatca gaatggcgtg gaaatgctca tggaaaatga aggaccccag
     2281 tcaggaacca acaagccaag ggaaacctgc cagggccctg agtgtcctgg cctccacacg
     2341 tttctcttgt ggtcccattc aggctttaac tgcctgcttt gtgcagagat gctgggacgg
     2401 aaagaggacc tcctccacca ctggaagcac cagcataact gtgaggaccc ttccaaactg
     2461 tgggctattt taaatacggt ctccaaccag ggagtgatcg aactttccag tgaagctgag
     2521 aaattgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     2581 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2641 tggctttata tatcttgtgg aaaggacgag atccatgtgg taggcttagg gcc
//
