LOCUS       sequence-286956-        1373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3471
FEATURES             Location/Qualifiers
     source          1..1373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1205..1229)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1237..1278
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg attcagtgtc atttgaagat
       61 gtggctgtgg cctttactca ggaggagtgg gctttgctgg atccttctca aaagaatctc
      121 tacagagatg tgatgcaaga aatcttcagg aacctggctt ctgtaggaaa caaatcagaa
      181 gaccagaata tccaagatga cttcaaaaat cctgggagaa atctaagcag tcatgtggta
      241 gagagactgt ttgaaattaa agaaggcagt caatatggag aaaccttcag ccaggattca
      301 aatttgaatc tgaataagaa agtttctact ggagtaaagc catgtgaatg cagtgtgtgt
      361 ggaaaagtct tcatatgtca ttcagccctt cataggcaca tcctgtctca cattggaaac
      421 aaactatttg agtgtgagga atgtccagag aagttatatc attgcaaaca atgtgggaaa
      481 gcctttatct ctctcaccag tgttgacaga cacatggtaa cacacactag taatgggcct
      541 tataagggtc cagtgtatga gaagcctttt gattttccta gtgtatttca aatgcctcag
      601 agcacttaca ctggagagaa aacatataaa tgtaaacatt gtgataaagc cttcaattat
      661 tcaagttatc ttcgtgaaca tgaaagaact catactggag agaaacccta tgcatgtaag
      721 aaatgtggta aatcattcac tttttccagt tctcttcgcc aacatgaaag atctcatact
      781 ggagagaaac cctatgaatg taaggaatgt ggcaaagcct tcagtcgttc cacttacttg
      841 ggaatacatg aaagaacgca tactggagaa aaaccctatg aatgtataaa atgtggcaaa
      901 gcctttagat gttccagagt cctcagagtc catgaaagga ctcacagtgg agaaaagccc
      961 tatgaatgta aacaatgtgg taaagccttc aaatattcta gtaacctatg tgagcatgaa
     1021 agaactcaca ctggagtgaa accttatgga tgtaaggaat gtggtaagtc gtttacttct
     1081 tccagtgccc ttcgaagcca tgaaaggact catactggag aaaaacccta tgaatgtaag
     1141 aaatgtggta aagccttcag ttgttccagt tcccttcgaa agcatgaaag agcttatatg
     1201 tggtacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1261 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1321 tggctttata tatcttgtgg aaaggacgaa tgcgcacagc tccgacgcat cgc
//
