LOCUS       sequence-286959-        3431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3472
FEATURES             Location/Qualifiers
     source          1..3431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3263..3287)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3295..3336
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ctactgcaaa ctgtggcgcc
       61 cacgacgagc tcgacttcaa actcgtcttt ggcgaggacg gggcgccggc gccgccgccc
      121 ccgggctcgc ggcctgcaga tcttgagcca gatgattgtg catccattta catctttaat
      181 gtagatccac ctccatctac tttaaccaca ccactttgct taccacatca tggattaccg
      241 tctcactctt ctgttttgtc accatcgttt cagctccaaa gtcacaaaaa ctatgaagga
      301 acttgtgaga ttcctgaatc taaatatagc ccattaggtg gtcccaaacc ctttgagtgc
      361 ccaagtattc aaattacatc tatctctcct aactgtcatc aagaattaga tgcacatgaa
      421 gatgacctac agataaatga cccagaacgg gaatttttgg aaaggccttc tagagatcat
      481 ctctatcttc ctcttgagcc atcctaccgg gagtcttctc ttagtcctag tcctgccagc
      541 agcatctctt ctaggagttg gttctctgat gcatcttctt gtgaatcgct ttcacatatt
      601 tatgatgatg tggactcaga gttgaatgaa gctgcagccc gatttaccct tggatcccct
      661 ctgacttctc ctggtggctc tccagggggc tgccctggag aagaaacttg gcatcaacag
      721 tatggacttg gacactcatt atcacccagg caatctcctt gccactctcc tagatccagt
      781 gtcactgatg agaattggct gagccccagg ccagcctcag gaccctcatc aaggcccaca
      841 tccccctgtg ggaaacggag gcactccagt gctgaagttt gttatgctgg gtccctttca
      901 ccccatcact cacctgttcc ttcacctggt cactccccca ggggaagtgt gacagaagat
      961 acgtggctca atgcttctgt ccatggtggg tcaggccttg gccctgcagt ttttccattt
     1021 cagtactgtg tagagactga catccctctc aaaacaagga aaacttctga agatcaagct
     1081 gccatactac caggaaaatt agagctgtgt tcagatgacc aagggagttt atcaccagcc
     1141 cgggagactt caatagatga tggccttgga tctcagtatc ctttaaagaa agattcatgt
     1201 ggtgatcagt ttctttcagt tccttcaccc tttacctgga gcaaaccaaa gcctggccac
     1261 acccctatat ttcgcacatc ttcattacct ccactagact ggcctttacc agctcatttt
     1321 ggacaatgtg aactgaaaat agaagtgcaa cctaaaactc atcatcgagc ccattatgaa
     1381 actgaaggta gccgaggggc agtaaaagca tctactgggg gacatcctgt tgtgaagctc
     1441 ctgggctata acgaaaagcc aataaatcta caaatgttta ttgggacagc agatgatcga
     1501 tatttacgac ctcatgcatt ttaccaggtg catcgaatca ctgggaagac agtcgctact
     1561 gcaagccaag agataataat tgccagtaca aaagttctgg aaattccact tcttcctgaa
     1621 aataatatgt cagccagtat tgattgtgca ggtattttga aactccgcaa ttcagatata
     1681 gaacttcgaa aaggagaaac tgatattggc agaaagaata ctagagtacg acttgtgttt
     1741 cgtgtacaca tcccacagcc cagtggaaaa gtcctttctc tgcagatagc ctctataccc
     1801 gttgagtgct cccagcggtc tgctcaagaa cttcctcata ttgagaagta cagtatcaac
     1861 agttgttctg taaatggagg tcatgaaatg gttgtgactg gatctaattt tcttccagaa
     1921 tccaaaatca tttttcttga aaaaggacaa gatggacgac ctcagtggga ggtagaaggg
     1981 aagataatca gggaaaaatg tcaaggggct cacattgtcc ttgaagttcc tccatatcat
     2041 aacccagcag ttacagctgc agtgcaggtg cacttttatc tttgcaatgg caagaggaaa
     2101 aaaagccagt ctcaacgttt tacttataca ccagttttga tgaagcaaga acacagagaa
     2161 gagattgatt tgtcttcagt tccatctttg cctgtgcctc atcctgctca gacccagagg
     2221 ccttcctctg attcagggtg ttcacatgac agtgtactgt caggacagag aagtttgatt
     2281 tgctccatcc cacaaacata tgcatccatg gtgacctcat cccatctgcc acagttgcag
     2341 tgtagagatg agagtgttag taaagaacag catatgattc cttctccaat tgtacaccag
     2401 ccttttcaag tcacaccaac acctcctgtg gggtcttcct atcagcctat gcaaactaat
     2461 gttgtgtaca atggaccaac ttgtcttcct attaatgctg cctctagtca agaatttgat
     2521 tcagttttgt ttcagcagga tgcaactctt tctggtttag tgaatcttgg ctgtcaacca
     2581 ctgtcatcca taccatttca ttcttcaaat tcaggctcaa caggacatct cttagcccat
     2641 acacctcatt ctgtgcatac cctgcctcat ctgcaatcaa tgggatatca ttgttcaaat
     2701 acaggacaaa gatctctttc ttctccagtg gctgaccaga ttacaggtca gccttcgtct
     2761 cagttacaac ctattacata tggtccttca cattcagggt ctgctacaac agcttcccca
     2821 gcagcttctc atcccttggc tagttcaccg ctttctgggc caccatctcc tcagcttcag
     2881 cctatgcctt accaatctcc tagctcagga actgcctcat caccgtctcc agccaccaga
     2941 atgcattctg gacagcactc aactcaagca caaagtacgg gccagggggg tctttctgca
     3001 ccttcatcct taatatgtca cagtttgtgt gatccagcgt catttccacc tgatggggca
     3061 actgtgagca ttaaacctga accagaagat cgagagccta actttgcaac cattggtctg
     3121 caggacatca ctttagatga tgtgaacgag ataattggga gagacatgtc ccagatttct
     3181 gtttcccaag gagcaggggt gagcaggcag gctcccctcc cgagtcctga gtccctggat
     3241 ttaggaagat ctgatgggct ctacccaact ttcttgtaca aagtggttga tatcggtaag
     3301 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     3361 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgactg gggcatatct
     3421 tgttacggac c
//
