LOCUS       sequence-28696-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQCXP CMV/TO RasV12 (w364-1)
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(341..365)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    406..424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    427..445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(447..650)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(476..496)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gacttggtgt tgttgatggc aaacacacac aggaagccct ccccggtgcg catgtactgg
       61 tcccgcatgg cgctgtactc ctcctggccg gcggtatcca ggatgtccaa caggcacgtc
      121 tccccatcaa tgaccacctg cttccggtag gaatcctcta tagtggggtc gtattcgtcc
      181 acaaaatggt tctggatcag ctggatggtc agcgcactct tgcccacacc gacggcgccc
      241 accaccacca gcttatattc cgtcatcgct cggccccggc cctggccccc caagctgtac
      301 caccacactg ggatccagtc gactgaattg gttcctttaa agcctgcttt tttgtacaaa
      361 cttgttgata tctgcagaat tccaccacac tggactagtc gacgatctct atcactgata
      421 gggagatctc tatcactgat agggagagct ctgcttatat agacctccca ccgtacacgc
      481 ctaccgccca tttgcgtcaa tggggcggag ntgttacgac attttggaaa gtcccgttga
      541 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
      601 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
      661 gcgatgacta atacgtanat gtactgccaa gtannaaagt cccataaggt catgtactgg
      721 gcataatgcc nngcgggcca ttttaccgtc att
//
