LOCUS       sequence-286962-        1649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3473
FEATURES             Location/Qualifiers
     source          1..1649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1481..1505)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1513..1554
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcttcaga cagcatattt
       61 gagtcatttc cttcgtaccc acagtgcttc atgagagaat gcatacttgg aatgaatcct
      121 tctagagacg tccacgatgc cagcacgagc cgccgcttca cgccgccttc caccgcgctg
      181 agcccaggca agatgagcga ggcgttgccg ctgggcgccc cggacgccgg cgctgccctg
      241 gccggcaagc tgaggagcgg cgaccgcagc atggtggagg tgctggccga ccacccgggc
      301 gagctggtgc gcaccgacag ccccaacttc ctctgctccg tgctgcctac gcactggcgc
      361 tgcaacaaga ccctgcccat cgctttcaag gtggtggccc taggggatgt tccagatggc
      421 actctggtca ctgtgatggc tggcaatgat gaaaactact cggctgagct gagaaatgct
      481 accgcagcca tgaagaacca ggttgcaaga tttaatgacc tcaggtttgt cggtcgaagt
      541 ggaagaggga aaagcttcac tctgaccatc actgtcttca caaacccacc gcaagtcgcc
      601 acctaccaca gagccatcaa aatcacagtg gatgggcccc gagaacctcg aagacatcgg
      661 cagaaactag atgatcagac caagcccggg agcttgtcct tttccgagcg gctcagtgaa
      721 ctggagcagc tgcggcgcac agccatgagg gtcagcccac accacccagc ccccacgccc
      781 aaccctcgtg cctccctgaa ccactccact gcctttaacc ctcagcctca gagtcagatg
      841 caggatacaa ggcagatcca accatcccca ccgtggtcct acgatcagtc ctaccaatac
      901 ctgggatcca ttgcctctcc ttctgtgcac ccagcaacgc ccatttcacc tggacgtgcc
      961 agcggcatga caaccctctc tgcagaactt tccagtcgac tctcaacggc acccgacctg
     1021 acagcgttca gcgacccgcg ccagttcccc gcgctgccct ccatctccga cccccgcatg
     1081 cactatccag gcgccttcac ctactccccg acgccggtca cctcgggcat cggcatcggc
     1141 atgtcggcca tgggctcggc cacgcgctac cacacctacc tgccgccgcc ctaccccggc
     1201 tcgtcgcaag cgcagggagg cccgttccaa gccagctcgc cctcctacca cctgtactac
     1261 ggcgcctcgg ccggctccta ccagttctcc atggtgggcg gcgagcgctc gccgccgcgc
     1321 atcctgccgc cctgcaccaa cgcctccacc ggctccgcgc tgctcaaccc cagcctcccg
     1381 aaccagagcg acgtggtgga ggccgagggc agccacagca actcccccac caacatggcg
     1441 ccctccgcgc gcctggagga ggccgtgtgg aggccctact tgccaacttt cttgtacaaa
     1501 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1561 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1621 gacgaatttc taggccacct cgttgagca
//
