LOCUS       sequence-286965-        2543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3474
FEATURES             Location/Qualifiers
     source          1..2543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2375..2399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2407..2448
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagctaatgg ggactctccc
       61 ccatggtccc cggccctggc tgcagaggga cgtggcagct catgtgaggc ttcagtgtca
      121 tttgaggacg tgactgtgga cttcagcaag gaggagtggc agcacttgga ccctgcccag
      181 agacgcctgt actgggatgt gacactagag aactacagcc acctgctctc agtggggtac
      241 caaattccca agtcagaggc tgccttcaag ttggagcaag gagaggggcc atggatgctg
      301 gagggggaag ccccacatca gagctgttca ggtgaggcta ttgggaaaat gcagcaacag
      361 ggaattcctg gaggaatttt cttccactgt gagagatttg atcaacccat aggagaagat
      421 tcattatgtt ctattttaga agaactgtgg caagataatg accagctaga gcaacgtcag
      481 gaaaaccaga ataacctttt aagtcatgtg aaagtattga ttaaggagag gggctatgaa
      541 cataaaaaca ttgaaaaaat aattcatgtg actaccaagc ttgttccttc aattaaaaga
      601 ctccataact gtgacacaat tttgaagcat actttaaact cacataatca taatagaaac
      661 agtgcaacaa agaaccttgg caagattttt ggaaatggta acaatttccc ccatagccct
      721 tcctctacta agaatgagaa tgctaaaaca ggagcaaatt cctgtgaaca tgaccactat
      781 gaaaaacatc tcagccacaa acaagctccc acccaccatc agaaaattca tcctgaggag
      841 aagctttatg tgtgtactga atgtgtaatg ggcttcactc agaagtcaca tctgtttgag
      901 catcagagaa ttcatgctgg agaaaagtcc cgtgaatgtg acaaaagcaa caaagtcttc
      961 ccccagaaac cccaggttga tgtacatcca agtgtttata caggagaaaa accctatctg
     1021 tgtactcaat gtgggaaagt ctttaccctc aaatcaaacc tcattacaca tcaaaaaatt
     1081 cataccgggc agaaacccta caaatgcagt gaatgtggaa aagccttttt ccagagatca
     1141 gacctcttta gacatctgag aattcataca ggagaaaaac cttatgaatg cagtgaatgt
     1201 ggaaaaggct tctcccagaa ctcagacctc agtatacatc agaaaactca taccggagag
     1261 aaacactatg aatgcaatga atgtgggaag gctttcacaa gaaaatcagc actcaggatg
     1321 catcagagaa tccacacggg agagaaacct tatgtatgcg ctgactgtgg gaaggccttc
     1381 atccagaaat cacatttcaa cacacatcag agaattcata ctggagaaaa gccgtatgaa
     1441 tgcagtgact gtgggaaatc cttcactaag aagtcacaac tccatgtgca tcaaagaatt
     1501 cacaccggag agaaacccta tatatgtaca gaatgtggaa aggtcttcac tcacaggaca
     1561 aacctcacca cacatcagaa aactcatact ggggaaaaac cctatatgtg tgctgaatgt
     1621 ggaaaggctt ttactgacca gtcaaatctc attaaacacc agaaaactca cactggagag
     1681 aaaccctata agtgcaatgg ctgtggaaaa gccttcatat ggaagtcgcg cctcaaaata
     1741 catcagaaat ctcatattgg agagagacac tatgaatgca aggactgcgg gaaagccttc
     1801 atccagaaat caacactaag cgtgcatcag agaatccata caggagagaa accgtacgtt
     1861 tgtcctgaat gcgggaaggc ctttatccag aaatcgcact tcattgcgca tcatagaatc
     1921 catactggag agaagcctta tgaatgcagc gactgtggga aatgcttcac taagaagtca
     1981 caactccgtg tgcatcagaa aatccacaca ggtgagaagc ccaatatatg tgctgaatgt
     2041 ggaaaggcct tcactgaccg atcaaatctc ataacacatc agaaaatcca cactagggag
     2101 aaaccctatg aatgtggtga ctgcgggaaa accttcacct ggaagtcacg cctcaatata
     2161 catcagaagt ctcatactgg agaaagacac tatgaatgta gtaaatgtgg gaaagctttc
     2221 atccagaaag ccacactaag tatgcatcag ataattcata caggaaagaa accttatgct
     2281 tgtacagaat gtcagaaggc ctttactgac agatcgaatc tcattaaaca ccagaaaatg
     2341 catagtggag aaaaacgcta taaagccagt gactgcccaa ctttcttgta caaagtggtt
     2401 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     2461 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2521 tgtaattagg gatcaagccc agc
//
