LOCUS       sequence-286968-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3475
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(617..641)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             649..690
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgctgaacc agtagaagac
       61 aattgcatca actttgtggc aatgaaattt attgacaata cgctttactt tatagctgaa
      121 gatgatgaaa acctggaatc cgattacttt ggcaagcttg aatctaaatt atcagtcata
      181 agaaatttga atgaccaagt tctcttcatt gaccaaggaa atcggcctct atttgaagat
      241 atgactgatt ctgactgtag agataatgca ccccggacca tatttattat aagtatgtat
      301 aaagatagcc agcctagagg tatggctgta actatctctg tgaagtgtga gaaaatttca
      361 actctctcct gtgagaacaa aattatttcc tttaaggaaa tgaatcctcc tgataacatc
      421 aaggatacaa aaagtgacat catattcttt cagagaagtg tcccaggaca tgataataag
      481 atgcaatttg aatcttcatc atacgaagga tactttctag cttgtgaaaa agagagagac
      541 ctttttaaac tcattttgaa aaaagaggat gaattggggg atagatctat aatgttcact
      601 gttcaaaacg aagactaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      661 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      721 atttcgattt cttggcttta tatatcttgt ggaaaggacg aagctgttcg acaaaggcat
      781 caaag
//
