LOCUS       sequence-286971-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3476
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(830..854)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             862..903
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgcgctcttt cctcgtcagg
       61 aagccctccg accccaatcg gaagcctaac tacagcgagc tgcaggactc taatccagag
      121 tttaccttcc agcagcccta cgaccaggcc cacctgctgg cagccatccc acctccggag
      181 atcctcaacc ccaccgcctc gctgccaatg ctcatctggg actctgtcct ggcgccccaa
      241 gcccagccaa ttgcctgggc ctcccttcgg ctccaggaga gtcccagggt ggcagagctg
      301 acctccctgt cagatgagga cagtgggaaa ggctcccagc cccccagccc accctcaccg
      361 gctccttcgt ccttctcctc tacttcagtc tcttccttgg aggccgaggc ctatgctgcc
      421 ttcccaggct tgggccaagt gcccaagcag ctggcccagc tctctgaggc caaggatctc
      481 caggctcgaa aggccttcaa ctgcaaatac tgcaacaagg aatacctcag cctgggtgcc
      541 ctcaagatgc acatccgaag ccacacgctg ccctgcgtct gcggaacctg cgggaaggcc
      601 ttctctaggc cctggctgct acaaggccat gtccggaccc acactggcga gaagcccttc
      661 tcctgtcccc actgcagccg tgccttcgct gaccgctcca acctgcgggc ccacctccag
      721 acccactcag atgtcaagaa gtaccagtgc caggcgtgtg ctcggacctt ctcccgaatg
      781 tccctgctcc acaagcacca agagtccggc tgctcaggat gtccccgctg cccaactttc
      841 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      901 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      961 tgtggaaagg acgactgagt agttctcata ctgttcga
//
