LOCUS       sequence-286974-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3477
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1121..1145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1153..1194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgggaca cctggcttca
       61 gattttgcct tctcgccccc tccaggtggt ggaggtgatg ggccaggggg gccggagccg
      121 ggctgggttg atcctcggac ctggctaagc ttccaaggcc ctcctggagg gccaggaatc
      181 gggccggggg ttgggccagg ctctgaggtg tgggggattc ccccatgccc cccgccgtat
      241 gagttctgtg gggggatggc gtactgtggg ccccaggttg gagtggggct agtgccccaa
      301 ggcggcttgg agacctctca gcctgagggc gaagcaggag tcggggtgga gagcaactcc
      361 gatggggcct ccccggagcc ctgcaccgtc acccctggtg ccgtgaagct ggagaaggag
      421 aagctggagc aaaacccgga ggagtcccag gacatcaaag ctctgcagaa agaactcgag
      481 caatttgcca agctcctgaa gcagaagagg atcaccctgg gatatacaca ggccgatgtg
      541 gggctcaccc tgggggttct atttgggaag gtattcagcc aaacgaccat ctgccgcttt
      601 gaggctctgc agcttagctt caagaacatg tgtaagctgc ggcccttgct gcagaagtgg
      661 gtggaggaag ctgacaacaa tgaaaatctt caggagatat gcaaagcaga aaccctcgtg
      721 caggcccgaa agagaaagcg aaccagtatc gagaaccgag tgagaggcaa cctggagaat
      781 ttgttcctgc agtgcccgaa acccacactg cagcagatca gccacatcgc ccagcagctt
      841 gggctcgaga aggatgtggt ccgagtgtgg ttctgtaacc ggcgccagaa gggcaagcga
      901 tcaagcagcg actatgcaca acgagaggat tttgaggctg ctgggtctcc tttctcaggg
      961 ggaccagtgt cctttcctct ggccccaggg ccccattttg gtaccccagg ctatgggagc
     1021 cctcacttca ctgcactgta ctcctcggtc cctttccctg agggggaagc ctttccccct
     1081 gtctctgtca ccactctggg ctctcccatg cattcaaact tgccaacttt cttgtacaaa
     1141 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgacattg ataaatccgg ttcgtagaa
//
