LOCUS       sequence-286977-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3478
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1358..1382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1390..1431
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaacttgcc aagagctgag
       61 cgccttcgct ccacaccgca gcgcagcctc cgggactccg atggggaaga cggtaaaatc
      121 gatgtcctgg gagaggagga agatgaagac gaggaggagg cggcgagcca gcagttccta
      181 gagcagtcgc tccagccggg gctgcaggtg gcccggtggg gcggggttgc gcttccccga
      241 gagcacatcg agggcggcgg cggcccgagc gacccctcag agtttggcac cgagttcagg
      301 gcaccgccaa ggtctgcggc ggcctctgaa gatgcccggc agccggcaaa gcccccctcc
      361 tcgtacatcg cgctcatcac catggccatc ctgcaaagcc cgcacaagcg cctcacgctc
      421 agcggcatct gcgccttcat tagtgaccgc ttcccctact accgccgcaa gttccccgcc
      481 tggcagaaca gcatccgcca caacctctcg ctgaacgact gcttcgtcaa gatcccccgc
      541 gagccgggcc gcccaggcaa gggcaactac tggagcctgg accccgcctc ccaggacatg
      601 ttcgacaatg gcagctttct ccggcgtagg aagcgtttcc agcgccacca accgaccccg
      661 ggagcccacc tgccccaccc cttccctcta cctgctgcac acgccgccct gcacaacccc
      721 cgcccaggcc ctctgcttgg ggcccctgcc ccgccgcagc cagtcccggg ggcctacccc
      781 aacaccggcc ccgggagacg cccttacgct ctgctgcacc cgcatcctcc tcgctaccta
      841 ctgctctcgg cccccgccta tgccggggca ccgaagaaag cagaaggcgc ggacctggcg
      901 accccggcac ccttcccgtg ctgcagccct cacttggtcc tcagccttgg gaggagggca
      961 agggtctggc gtcgccaccg ggaggcggat gcatctcttt cagcattgag agtatcatgc
     1021 aaggggtcag gggagcgggt acaggggctg cgcagagttt gtccccgacc gcgtggagct
     1081 actgccccct gctccagcga ccgtcaagcc tgtcggacaa ttttgcagca acagcagcgg
     1141 catcaggagg aggactgcgc caacggctgc gctcccacca agggcgcggt gctgggcggg
     1201 cacctgtcgg ccgcgtcggc gctgctgcgg tatcaggcgg tggcagaggg ctctgggctg
     1261 acatcgctgg ccgccccttt gggcggagag gggacctcac cagttttttt agtatcgccc
     1321 acgcccagtt ccctggccga gtccgcaggg ccctccttgc caactttctt gtacaaagtg
     1381 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gacccaccct aacgagctcg aatcca
//
