LOCUS       sequence-286980-        2327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3479
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2159..2183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2191..2232
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggccctgcc cagcccacag
       61 gttctaatgg ggttgccagc gttgctgatg ggcccggcgc agcatacttc ttggccctgt
      121 ggctcagctg tccccacatt gaagagcatg gtaacctttg aagatgtggc tgtatacttc
      181 tcccaagagg agtgggagct ccttgatgca gcccagaggc acctgtacca cagtgtgatg
      241 ctggaaaacc ttgagcttgt gacctcactt ggttcttggc atggagtaga gggtgagggg
      301 gcccatccca agcagaatgt ttctgtagaa gtgttacagg tcaggatccc taatgcagat
      361 ccttccacca agaaagctaa ctcctgtgac atgtgtgggc cattcttgaa agacattttg
      421 cacctggctg agcatcaggg aacacagtct gaggagaaac cctacacatg tggagcatgt
      481 gggagagact tttggttgaa tgcaaacctt caccagcacc agaaggagca cagtggaggg
      541 aagcccttta gatggtacaa ggacagggac gcacttatga agagctctaa agtccacctg
      601 tcagagaacc ccttcacttg cagggaaggt gggaaggtca tcctgggcag ctgtgacctc
      661 ctccagcttc aagctgttga cagtgggcag aagccatatt ccaatcttgg gcagcttcca
      721 gaagtctgta ccacacagaa actcttcgag tgcagcaact gtggaaaagc cttcctgaag
      781 agctccactc tccccaacca tctgagaact cactctgaag agataccatt tacatgccca
      841 acaggtggaa atttcttaga ggagaaatca atccttggta ataaaaaatt tcacactggg
      901 gaaatacccc atgtgtgtaa ggagtgtggg aaggccttta gtcactcatc taagctgagg
      961 aagcaccaga aatttcacac tgaagtaaaa tattatgagt gcattgcatg tgggaaaacc
     1021 ttcaaccaca aactcacatt tgttcatcat cagagaattc actcaggaga aagaccttat
     1081 gagtgtgatg aatgtgggaa agccttcagt aacagatcac acctcattcg gcatgagaaa
     1141 gttcacactg gagaaaggcc ttttgagtgc ctgaaatgtg gaagagcctt cagccaaagc
     1201 tccaatttcc ttcggcatca gaaagttcac acacaggtaa gaccttatga gtgcagtcaa
     1261 tgtggtaaat ccttcagccg aagctctgct ctcattcagc actggagagt tcacactgga
     1321 gaaagaccgt atgaatgcag tgaatgtgga agagctttta acaataactc caaccttgct
     1381 cagcaccaga aagttcacac cggagaacgg ccttttgagt gcagtgaatg tggaagagac
     1441 ttcagccaaa gctcccatct ccttcgacat cagaaagttc acactggaga acggcctttt
     1501 gaatgctgtg attgtggtaa agccttcagt aatagctcca ccctcatcca gcaccagaaa
     1561 gtacatactg ggcaaaggcc ttatgagtgc agcgaatgta ggaaatcctt cagccgcagc
     1621 tccagcctga ttcagcactg gagaattcac actggagaaa agccttacga gtgtagtgag
     1681 tgtgggaaag cctttgctca cagctccact ctcattgaac actggagagt tcacacaaaa
     1741 gaaaggcctt atgagtgcaa tgaatgtggg aaattcttta gccaaaactc cattctcatt
     1801 aagcatcaga aagttcatac tggagaaaag ccttataaat gcagtgaatg tgggaaattc
     1861 tttagccgaa aatccagcct tatttgtcac tggagagttc acactggaga aaggccttac
     1921 gaatgcagtg aatgtgggag agcctttagc agtaactccc acctggttcg tcatcagaga
     1981 gttcacacac aagaaaggcc ctatgagtgc atccagtgtg gaaaagcctt tagtgaaaga
     2041 tctacacttg ttcggcacca gaaagttcac accagagaaa ggacttatga gtgtagccag
     2101 tgtgggaaac tcttcagcca tctttgtaac cttgcacagc ataaaaagat tcataccttg
     2161 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2221 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2281 tatatatctt gtggaaagga cgaggttgcc aaggagctta tcaccag
//
