LOCUS       sequence-286983-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3480
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1253..1277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1285..1326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ggccgcccct ggcgccgcgc
       61 ccggcccacg tacccgggga ggccgggccc cggaggacga gggaaagcag gccgggcgcc
      121 gtgagcttcg cggacgtggc cgtgtacttc tctcccgagg agtgggaatg cctgcggcca
      181 gcgcagaggg ccctgtaccg ggacgtgatg cgggagacct tcggccacct gggcgcgctg
      241 ggattttcag ttcccaaacc cgcttttatc tcgtgggtgg aaggagaagt ggaggcgtgg
      301 agcccggagg cccaggatcc cgacggtgag agctctgcag ctttcagcag gggccaagga
      361 caggaagcag gatccaggga tgggaatgag gagaaggaaa ggctgaagaa gtgtccaaaa
      421 caaaaagagg tggcgcatga agtggctgtc aaggagtggt ggcccagcgt cgcctgccca
      481 gagttctgca accctaggca gagccccatg aatccctggc tcaaggacac tctgacccga
      541 agactgcccc actcttgccc agactgtggc cgcaacttca gctacccttc cctcctggcc
      601 agccaccagc gggtccactc cggggagcgg cccttctcct gcggccagtg tcaggcgcgt
      661 ttctcccagc gcaggtacct gctccagcat cagttcatcc acaccggcga gaagccctac
      721 ccctgccccg actgcgggcg ccgcttccgc cagaggggtt ccctggctat ccacaggcgg
      781 gctcacaccg gggagaagcc ttacgcgtgc tcagactgca agagtcgctt cacttacccc
      841 tacctgctgg ccatccacca gcgcaagcac acgggcgaga agccctacag ctgccccgat
      901 tgcagcctcc gtttcgccta cacctccctg ctggccatcc acaggcgcat acacaccggc
      961 gagaagccct acccctgtcc tgactgcggc cgccgcttca cctattcttc cctcctcctc
     1021 agtcaccggc gcattcactc cgacagccgg cccttcccct gcgtggagtg tgggaaaggc
     1081 ttcaagcgca agaccgccct ggaagcccat cggtggatcc accgctcctg cagcgagagg
     1141 cgcgcgtggc agcaggccgt ggtggggcgt tcagagccca tccctgtttt gggaggcaag
     1201 gatcccccag ttcacttccg gcactttcca gatatatttc aagagtgtgg gtgcccaact
     1261 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1321 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgacca gagtgtgtca cgttcctgag g
//
