LOCUS       sequence-286995-        2132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3484
FEATURES             Location/Qualifiers
     source          1..2132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1964..1988)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1996..2037
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgaggcggc cccggccccg
       61 acatctgaat gggactccga gtgccttaca tccctgcagc cccttcctct tcctacaccc
      121 ccagcagcaa atgaggcaca cctgcagaca gcagctatct ctctgtggac agtggtggcc
      181 gccgtgcagg ctatagagag gaaggtggag atccacagcc ggcgactcct acacctagaa
      241 ggtcggacag ggacagcaga gaagaaacta gccagctgtg aaaagacagt taccgagctt
      301 gggaaccagc tggagggcaa gtgggccgtg ctgggaaccc tgctgcagga gtacgggctg
      361 ctgcagaggc ggctggagaa cttggagaac ctgctgcgca acaggaactt ctggatcctg
      421 cggctccctc caggtattaa gggagatatc ccaaaggtgc ctgtggcatt tgatgatgtc
      481 tccatctact tttccactcc agagtgggaa aaattagaag aatggcaaaa ggaactttac
      541 aagaatatca tgaagggcaa ctacgagtct ctcatctcca tggattatgc tataaatcaa
      601 cctgatgtct tatctcagat tcaaccagaa ggggaacata atacagagga ccaggcaggg
      661 ccagaggaaa gtgagattcc cacagacccc agtgaagagc ctggtatttc aacatcagat
      721 attctgtctt ggattaaaca agaagaagag cctcaggttg gggccccacc ggagtccaag
      781 gagagtgacg tgtacaaaag cacttatgct gatgaagagc ttgtcatcaa agctgaaggc
      841 cttgctagat cctcgttgtg ccctgaggtt ccagtccctt tctcttctcc accagcagca
      901 gcaaaggatg ctttttcaga tgtggctttc aaaagccagc agtctacatc catgacacct
      961 tttggacgtc cagccactga cctgcctgaa gcctctgagg gacaagtgac ttttactcag
     1021 ttgggtagct atcccctccc acctccagtt ggcgagcagg tgttctcatg ccaccactgt
     1081 ggcaagaatc tcagccaaga catgttgctg acccaccaat gtagccatgc tactgagcac
     1141 cccttaccct gtgcccagtg ccctaagcac tttactccac aggcggacct cagcagcacc
     1201 tcccaggacc atgccagcga gacacccccc acctgcccac actgtgccag gacttttact
     1261 cacccatcaa gacttaccta ccatcttcgg gtccataaca gcactgagcg tcctttcccc
     1321 tgtcctgatt gccccaagcg ctttgctgac caggctcgac tcaccagcca ccggagagct
     1381 catgcaagcg aaaggccctt ccgctgtgcc cagtgcggca ggagcttcag cttgaaaatc
     1441 agcctcctgc tccaccagcg gggtcatgca caagagcgcc ctttctcctg ccctcagtgt
     1501 ggcattgact tcaacggcca ctcggccctg atccgccacc agatgatcca cacaggcgag
     1561 cgtccttacc cctgcactga ctgcagtaag agcttcatgc gcaaggagca cctgctgaac
     1621 caccggcggc tgcacacagg cgagcggccc ttcagttgtc ctcactgtgg caagagcttc
     1681 atccgcaagc accacctaat gaaacaccag cgcatccaca ccggggagcg gccctacccc
     1741 tgctcctact gtggcaggag cttccgctac aaacagacac tcaaggacca cctccgttca
     1801 ggccacaatg gaggctgtgg gggtgatagt gacccatcag gtcagccacc caacccacca
     1861 ggtcccctca taactgggct tgaaacttct ggcctgggtg tcaacactga aggtctagag
     1921 accaaccagt ggtatgggga agggagtgga gggggagttt tgtacccaac tttcttgtac
     1981 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     2041 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2101 aaggacgatc actattttta tccgagatca gt
//
