LOCUS       sequence-286998-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3485
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1556..1580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1588..1629
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaaattcaa gttacatgtg
       61 aattctgcca ggcaatacaa ggacctgtgg aatatgagtg atgacaaacc ctttctatgt
      121 actgcgcctg gatgtggcca gcgttttacc aacgaggatc atttggctgt ccataaacat
      181 aaacatgaga tgacactgaa atttggtcca gcacgtaatg acagtgtcat tgtggctgat
      241 cagaccccaa caccaacaag attcttgaaa aactgtgaag aagtgggttt gtttaatgag
      301 ttggcgagtc catttgagaa tgaattcaag aaagcttcag aagatgacat taaaaaaatg
      361 cctctagatt tatcccctct tgcaacacct atcataagaa gcaaaattga ggagccttct
      421 gttgtagaaa caactcacca ggatagtcct ttacctcacc cagagtctac taccagtgat
      481 gagaaggaag taccattggc acaaactgca cagcccacat cagctattgt tcgtccagca
      541 tcattacagg ttcccaatgt gctgcttaca agttctgact caagtgtaat tattcagcag
      601 gcagtacctt caccaacctc aagtactgta atcacccagg caccatcctc taacaggcca
      661 attgtccctg taccaggccc atttcctctt ctgttacatc ttcctaatgg acaaaccatg
      721 cctgttgcta ttcctgcatc aattacaagt tctaatgtgc atgttccagc tgcagtccca
      781 ctcgttcgac cagtcaccat ggtgcctagt gttccaggaa tcccaggtcc ttcctctccc
      841 caaccagtac agtcagaagc aaaaatgaga ttaaaagctg ctttgaccca gcaacatcct
      901 ccagttacca atggtgatac tgtcaaaggt catggtagcg gattggttag gactcagtca
      961 gaggaatctc gaccgcagtc attacaacag ccagccacat ccactacaga aactccggct
     1021 tctccagctc acacaactcc acagacccaa agtacaagtg gtcgtcggag aagagcagct
     1081 aacgaagatc ctgatgaaaa aaggagaaag tttttagagc gaaatagagc agcagcttca
     1141 agatgccgac aaaaaaggaa agtctgggtt cagtctttag agaagaaagc tgaagacttg
     1201 agttcattaa atggtcagct gcagagtgaa gtcaccctgc tgagaaatga agtggcacag
     1261 ctgaaacagc ttcttctggc tcataaagat tgccctgtaa ccgccatgca gaagaaatct
     1321 ggctatcata ctgctgataa agatgatagt tcagaagaca tttcagtgcc gagtagtcca
     1381 catacagaag ctatacagca tagttcggtc agcacatcca atggagtcag ttcaacctcc
     1441 aaggcagaag ctgtagccac ttcagtcctc acccagatgg cggaccagag tacagagcct
     1501 gctctttcac agatcgttat ggctccttcc tcccagtcac agccctcagg aagtttgcca
     1561 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1621 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1681 atatcttgtg gaaaggacga tcgcttttgt cgagagagtt tccg
//
