LOCUS       sequence-287001-        2282 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3486
FEATURES             Location/Qualifiers
     source          1..2282
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2114..2138)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2146..2187
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaaaagat ctattccaga
       61 ggggagcttc accacttcat tgacggcttt aatgaagaga aaagcaactg gatgcgctat
      121 gtgaatccag cacactctcc ccgggagcaa aacctggctg cgtgtcagaa cgggatgaac
      181 atctacttct acaccattaa gcccatccct gccaaccagg aacttcttgt gtggtattgt
      241 cgggactttg cagaaaggct tcactaccct tatcccggag agctgacaat gatgaatctc
      301 acacaaacac agagcagtct aaagcaaccg agcactgaga aaaatgaact ctgcccaaag
      361 aatgtcccaa agagagagta cagcgtgaaa gaaatcctaa aattggactc caacccctcc
      421 aaaggaaagg acctctaccg ttctaacatt tcacccctca catcagaaaa ggacctcgat
      481 gactttagaa gacgtgggag ccccgaaatg cccttctacc ctcgggtcgt ttaccccatc
      541 cgggcccctc tgccagaaga ctttttgaaa gcttccctgg cctacgggat cgagagaccc
      601 acgtacatca ctcgctcccc cattccatcc tccaccactc caagcccctc tgcaagaagc
      661 agccccgacc aaagcctcaa gagctccagc cctcacagca gccctgggaa tacggtgtcc
      721 cctgtgggcc ccggctctca agagcaccgg gactcctacg cttacttgaa cgcgtcctac
      781 ggcacggaag gtttgggctc ctaccctggc tacgcacccc tgccccacct cccgccagct
      841 ttcatcccct cgtacaacgc tcactacccc aagttcctct tgccccccta cggcatgaat
      901 tgtaatggcc tgagcgctgt gagcagcatg aatggcatca acaactttgg cctcttcccg
      961 aggctgtgcc ctgtctacag caatctcctc ggtgggggca gcctgcccca ccccatgctc
     1021 aaccccactt ctctcccgag ctcgctgccc tcagatggag cccggaggtt gctccagccg
     1081 gagcatccca gggaggtgct tgtcccggcg ccccacagtg ccttctcctt taccggggcc
     1141 gccgccagca tgaaggacaa ggcctgtagc cccacaagcg ggtctcccac ggcgggaaca
     1201 gccgccacgg cagaacatgt ggtgcagccc aaagctacct cagcagcgat ggcagccccc
     1261 agcagcgacg aagccatgaa tctcattaaa aacaaaagaa acatgaccgg ctacaagacc
     1321 cttccctacc cgctgaagaa gcagaacggc aagatcaagt acgaatgcaa cgtttgcgcc
     1381 aagactttcg gccagctctc caatctgaag gtccacctga gagtgcacag tggagaacgg
     1441 cctttcaaat gtcagacttg caacaagggc tttactcagc tcgcccacct gcagaaacac
     1501 tacctggtac acacgggaga aaagccacat gaatgccagg tctgccacaa gagatttagc
     1561 agcaccagca atctcaagac ccacctgcga ctccattctg gagagaaacc ataccaatgc
     1621 aaggtgtgcc ctgccaagtt cacccagttt gtgcacctga aactgcacaa gcgtctgcac
     1681 acccgggagc ggccccacaa gtgctcccag tgccacaaga actacatcca tctctgtagc
     1741 ctcaaggttc acctgaaagg gaactgcgct gcggccccgg cgcctgggct gcccttggaa
     1801 gatctgaccc gaatcaatga agaaatcgag aagtttgaca tcagtgacaa tgctgaccgg
     1861 ctcgaggacg tggaggatga catcagtgtg atctctgtag tggagaagga aattctggcc
     1921 gtggtcagaa aagagaaaga agaaactggc ctgaaagtgt ctttgcaaag aaacatgggg
     1981 aatggactcc tctcctcagg gtgcagcctt tatgagtcat cagatctacc cctcatgaag
     2041 ttgcctccca gcaacccact acctctggta cctgtaaagg tcaaacaaga aacagttgaa
     2101 ccaatggatc ctttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2161 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2221 tcgatttctt ggctttatat atcttgtgga aaggacgacg tcgggtcctc gactctacat
     2281 ta
//
