LOCUS       sequence-287007-        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3488
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1808..1832)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1840..1881
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccttccggga tgtggctgtg
       61 gccttcaccc agaaggagtg gaagctattg agttctgctc agaggaccct gtacagggag
      121 gtgatgctgg agaactacag ccatctggtc tccctgggaa ttgcattttc caaaccaaaa
      181 ctcatcgaac agctggagca aggcgacgaa ccttggagag aggagaacga acatcttctg
      241 gacctttgtc cagagcctag aacagaattc cagccaagtt ttccccacct ggtggccttt
      301 tctagctcgc agctcctcag acaatatgcg ctaagtggcc atcccacaca gatcttccca
      361 agctcatctg caggaggtga cttccaacta gaagctccaa gatgctctag tgaaaaagga
      421 gaaagtggag agacagaagg ccccgacagc tcattaagaa agaggccaag cagaatttct
      481 aggacattct tcagcccaca tcaaggtgac ccagtagaat gggtagaagg gaacagagaa
      541 ggaggaacag accttcgcct ggcccaaagg atgagtcttg gggggtcaga cacaatgttg
      601 aagggagcag acacttcaga atctggagca gtcatacgtg gaaactatag actgggactt
      661 agcaaaaagt caagcctgtt cagccaccag aagcatcatg tgtgccctga atgcgggaga
      721 ggcttttgcc agagatcaga ccttatcaag caccagagga cacacaccgg ggagaagcca
      781 tacctgtgtc ctgagtgtgg gcgtcggttt agccagaagg cctccctctc catacaccag
      841 aggaagcact cgggggagaa gccgtatgtg tgcagggaat gtgggcgaca cttcaggtat
      901 acatcctctc tcactaatca caagaggatt cactccgggg agaggccctt tgtatgtcag
      961 gagtgtgggc gaggctttcg ccagaagata gccctccttc tacaccagag gacgcacttg
     1021 gaggagaagc ccttcgtgtg tcctgagtgt gggagaggct tttgccagaa ggcatcactc
     1081 ctccagcacc agagctcaca cacaggggag aggcccttcc tgtgccttga gtgtgggcgt
     1141 agcttcaggc agcagtcact cctccttagt caccaggtca cacactcagg agagaagcct
     1201 tatgtctgtg ctgagtgtgg gcacagcttt cgccaaaagg tcactctcat caggcaccag
     1261 aggacacaca caggggagaa gccttacctg tgcccccagt gtgggcgggg ttttagccag
     1321 aaggtcaccc tcattggaca ccagaggaca cacacagggg agaagcccta cctgtgccct
     1381 gattgtgggc gtggctttgg tcagaaggtc accctcatca gacaccagag gacacacaca
     1441 ggggagaagc cttatctgtg ccccaagtgt gggcgtgcat ttggctttaa gtcgctcctc
     1501 acccgacacc agaggacaca ctcagaggag gagctttacg tagacagggt gtgtggacaa
     1561 ggacttggcc agaagtcaca ccttatctct gaccaaagga cacactcagg agagaagccc
     1621 tgcatttgcg atgaatgtgg gcgcggcttt ggctttaagt ctgccctcat ccgacatcag
     1681 cggacccatt ctggggagaa gccgtatgtc tgcagggagt gtgggcgtgg ctttagccag
     1741 aagtctcact tgcatagaca caggaggacc aagtctggtc atcagctcct accccaagag
     1801 gtcttctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1861 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1921 tcttggcttt atatatcttg tggaaaggac gacggtaaaa ccactatttg gcccgc
//
